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NOD1 Stable Knockout AGS (41A8) Cell Line

SKU : BHC10901368

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Applied Biological Materials (abm) Inc.

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Review(s)

Mfr.No.	T6187
Description	CRISPR/Cas9 gene editing was used to generate NOD1 KO clones. AGS cells were transformed with vectors expressing Cas9 nickase nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene. NOD1 Stable Knockout AGS (41A8) Cell Line (available at abm; Cat. No. T6187) and NOD1 Stable Knockout AGS (41H8) Cell Line were confirmed as having 71-bp and 31-bp deletions within the coding regions of the caspase activation and recruitment domain (CARD) of NOD1. This cell line had been shown to have defective CXCL8 responses to stimulation with NOD1 ligands. Control cells are also available abm; Cat. No. T8246 to use alongside with the cells.
Cell Type	Drug Discovery Cell Lines
Disclaimer	1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item. 2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 mug, Cat.# C207, $450.00) or cell lysate (100 mug, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Expression Level	Puromycin resistance
Quantity	1x106 cells / 1.0 ml
Tissue	Stomach
Morphology	Epithelial-like
Species	Human (H. sapiens)
Quality Control	1) PCR; 2) Pyrosequencing

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SKU	Supplier No.	Your price	Quantity
BHC10901368	T6187	$3950		Add to Cart

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