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Home / PCR, qPCR & RT-PCR / PCR

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based

SKU : BHR10900074

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Applied Biological Materials (abm) Inc.

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Mfr.No.	G476
Description	Second Strand cDNA Synthesis Kit is an efficient system of generating double stranded cDNA from first strand cDNA as a template. The E. coli RNase H nicks the RNA in the DNA:RNA hybrid, while the E. coli DNA Polymerase replaces the RNA with deoxyribonucleotides. The E. coli DNA Ligase completes the double stranded DNA formation by linking the gaps between the newly synthesized cDNA strand. The dNTP based kit (Cat. No. G475) and dNTP/dUTP based kit (Cat. No. G476) provides different combination of deoxyribonucleotides tailored to the end user’s needs and application. This kit is provided as individual enzymes to meet the customer’s needs and to provide maximum efficiency and flexibility in the RNA sample preparation. (Noted that these enzymes may still contain E. coli gDNA) The double stranded cDNA end product can subsequently be converted to blunt ended DNA fragments using abm’s DNA End Repair Kit (Cat. No. G477), followed by abm’s dA Tailing Kit (Cat. No. E009) to generate DNA suitable for whole genome sequencing.
Template	RNA
Description	RNA-Seq Library Construction. downstream double-stranded blunt-end cDNA synthesis for cloning. downstream double-stranded cDNA library construction.
Format	Kit
Quantity	25 x 50 µl reactions
Reaction	RT-PCR

Reference	Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR. Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR. Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription. Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.

Storage Condition	-20C
Shipping Condition	Dry Ice
Storage	Store all components at -20°C. All components are stable for 1 year from the date of shipping when stored and handled properly. Avoid repeated freeze-thaw cycles to retain maximum performance.

Other Download	Protocol PCR RT-PCR qPCR Application Handbook Real-Time PCR Set-up instructions (for abm Reverse Transcription Products) Version 1.2
MSDS	10X Second Strand cDNA Synthesis Buffer
Others	dNTP/dUTP Mixture (10mM)

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SKU	Supplier No.	Size	Your price	Quantity
BHR10900074	G476	25x50µl-reactions	$455		Add to Cart

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