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Home / Biochemicals / Small Molecules

PhTx-74

SKU : BHB21300264

Selective Antagonist of Various AMPA Receptors

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Mfr.No.	P-120, P-120_1 mg, P-120_10 mg, P-120_25 mg, P-120_5 mg, P-120_50 mg
Description	PhTx-74 (#P-120) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C24H44Cl2N4O3.
Synonyms	Synthetic
Background	PhTx-74 (Philanthotoxin-7,4) is a synthetic polyamine analogue of the naturally occurring wasp venom toxin philanthotoxin-4,3,3. It is a potent and selective antagonist of certain isoforms of the AMPA type of ionotropic glutamate receptors1.PhTx-74 inhibited GluR1 and GluR3 homomers as well as GluR1/GluR2 where 100 uM inhibited nearly 100% of the glutamate evoked currents. However, up to 500 uM had no or little effect on GluR2 or GluR2/GluR3 channels1-2.PhTx-74 (5 uM) was used as a tool to demonstrate circadian changes in AMPAR subtypes functionally expressed in somatosensory cortex slices and the removal of some AMPAR during sleep.Indeed, EPSPs were significantly depressed by PhTx-74 only after dark episodes3. As a measure for changes in AMPAR subunit composition after cocaine administration, PhTx-74 was used in synaptic transmission evaluation. AMPAR EPSCs from neurons treated earlier with cocaine showed increased sensitivity to bath application of 100 uM PhTx-74 compared with vehicle-treated cells, suggesting an increased contribution of GluR1-containing AMPARs to synaptic transmission after cocaine4.PhTx-74 was used to evaluate the effects of transmembrane AMPAR regulatory proteins (TARPs) on AMPAR conductance; 100 uM PhTx-74 blocked GluA2/A4 currents to a different degree depending on the auxiliary TARP present5.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	0.1-100 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Cas Number	401601-12-5
Molecular Weight	507.54
Solubility	Water. Centrifuge all product preparations before use (10000 x g 5 min).
Target	AMPA receptors
Activity	PhTx-74 (Philanthotoxin-7,4) is a synthetic polyamine analogue of the naturally occurring wasp venom toxin philanthotoxin-4,3,3. It is a potent and selective antagonist of certain isoforms of the AMPA type of ionotropic glutamate receptors1. PhTx-74 inhibited GluR1 and GluR3 homomers as well as GluR1/GluR2 where 100 uM inhibited nearly 100% of the glutamate evoked currents. However, up to 500 uM had no or little effect on GluR2 or GluR2/GluR3 channels1-2.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: (S)-N-(7-((4-Aminobutyl)amino)heptyl)-4-hydroxy-alpha-((1-oxobutyl)amino)benzenepropanamide dihydrochloride.

Purity	>99% (HPLC)
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to one week at 4°C or six months at -20°C.

Product Manual	SDS

Reference	Kromann, H. et al. (2002) J. Med. Chem. 45, 5745. Nilsen, A. and England, P.M. (2007) J. Am. Chem. Soc. 129, 4902. Lante, F. et al. (2011) J. Neurosci. 31, 3953. Argilli, E. et al. (2008) J. Neurosci. 28, 9092. Jackson, A.C. et al. (2011) J. Neurosci. 31, 7511. Kromann, H. et al. (2002) J. Med. Chem. 45, 5745. Nilsen, A. and England, P.M. (2007) J. Am. Chem. Soc. 129, 4902.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300264-1	P-120_1 mg	1 mg	$55		Add to Cart
BHB21300264-2	P-120_10 mg	10 mg	$129		Add to Cart
BHB21300264-3	P-120_25 mg	25 mg	$261		Add to Cart
BHB21300264-4	P-120_5 mg	5 mg	$91		Add to Cart
BHB21300264-5	P-120_50 mg	50 mg	$448		Add to Cart

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