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Home / Primary Antibodies / Polyclonal Antibodies

Anti-CD9 (extracellular)-APC Antibody

SKU : BHA21300782

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Mfr.No.	ANR-209-APC, ANR-209-APC_15 mcl, ANR-209-APC_50 mcl
Lead Time	1-2 Business Days
Immunogen Species	Mouse
Background	Cluster of Differentiation 9 (CD9), also known as Tetrapanin-29, TSPAN29 and Leukocyte Antigen MIC3, is a member of the tetraspanin superfamily, a group of proteins characterized by four transmembrane domains, two extracellular loops, and N- and C- termini cytoplasmic tails.1The tetraspanins family members function as organizers of the cell surface by recruiting specific partner proteins into tetraspanin-enriched microdomains. Thus, depending on the recruited protein, they influence many cellular activities in different cells, including intracellular signaling, proliferation, activation, survival, migration, invasion, adhesion, and diapedesis.1CD9 was originally identified as a lymphohematopoietic marker, and is indeed expressed in all the major subsets of leukocytes, however, it is also widely expressed across different cell populations including endothelial cells, neurons, glia and oocytes.2,3CD9 plays various roles in the immune system, it has been implicated in T cell activation and participating in the formation of immunological synapses by interacting with the integrin LFA-1 (on the T-cell side) and their ligand, the integrin ICAM-1 on the antigen presenting cell (APC) surface.2,3 In B cells, CD9 plays a role in B cell activation and regulation of antibody production, and in mast cells is involved in the regulation of signalling pathways that influence the release of allergic mediators in response to allergens.2,3CD9 has complex roles in the central nervous system (CNS). It has been implicated in neurite outgrowth and cell migration in association with the neural adhesion molecule L1 (L1CAM). It is also involved in myelination and the maintaining of the myelin sheath, as well as the regulation of neural progenitor cells.4Finally, CD9 is often associated with exosomes, small extracellular vesicles released by cells that play a crucial role in intercellular communication by carrying proteins, lipids, and nucleic acids between cells. CD9 is considered a marker for exosomes and detection of CD9 on the surface of extracellular vesicles is often used as a method to isolate and identify exosomes from biological fluids or cell culture supernatants.5,6
Clonality	Polyclonal
Description	Anti-CD9 (extracellular) Antibody (#ANR-209) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD9 from mouse, rat and human samples. Anti-CD9 (extracellular)-APC Antibody (#ANR-209-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
Formulation	PBS pH7.4, 1% BSA with 0.05% sodium azide
Host	Rabbit
Immunogen Region	Extracellular, 2nd loop.
Isotype	Rabbit IgG
Reactivity	Human, Rat, Mouse
Reconstitution	15 ul or 50 ul double distilled water (DDW), depending on the sample size.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Synonyms	Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3
UNSPSC Code	41116161
Note	Homology: Rat - identical, Human - 14 out of 16 amino acid residues identical Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry Negative Control: RIC-001-APC Specifictiy: CD9 Is Toxin: No Standard quality control of each lot: Western blot analysis (unlabeled antibody, #ANR-209), and direct flow cytometry (labeled antibody). Antigen preadsorption control: 1 ug peptide per 1 ug antibody

Accession Number	P40240
Gene Id	12527
Target	Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3
Sequence	(C)EVIKELQEFYKDTYQK, corresponding to amino acid residues 114 - 129 of mouse CD9

Conjugates	Allophycocyanin (APC).

Concentration	1 mg/ml
Form	Lyophilized powder
Preservative	1% BSA, 0.05% NaN3
Purity	Affinity purified on immobilized antigen.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Applications	FC, LCI
Dilution	Contact Alomone for information

Product Manual	SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA21300782-1	ANR-209-APC_15 mcl	15 mcl	$597		Add to Cart
BHA21300782-2	ANR-209-APC_50 mcl	50 mcl	$797		Add to Cart
BHA21300782-3	ANR-209-APC_50 mcl	2x50 mcl	$1397		Add to Cart
BHA21300782-4	ANR-209-APC_50 mcl	5x50 mcl	$2743		Add to Cart
BHA21300782-5	ANR-209-APC_50 mcl	10x50 mcl	$3764		Add to Cart

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