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Home / Primary Antibodies / Polyclonal Antibodies

Anti-ch TOG/CKAP5 Antibody Picoband®

SKU : BHA21002214

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Mfr.No.	A05324, A05324-10ug, A05324-Biotin, A05324-Cy3, A05324-Dylight488, A05324-Dylight550, A05324-Dylight594, A05324-FITC, A05324-HRP, A05324-APC, A05324-PE, A05324-iFluor647, A05324-carrier-free
Alternative Names	cytoskeleton associated protein 5
Background	Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene. It is mapped to 11p11.2. This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for ional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacternatively spliced transcript variants encoding different isoforms have been identified.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-ch TOG/CKAP5 Antibody Picoband® catalog # A05324. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. I
Host	Rabbit
Immunogen Region	E.coli-derived human ch TOG/CKAP5 recombinant protein (Position: M1-L221).
Isotype	Rabbit IgG
Molecular Weight	225 kDa
Reactivity	Human, Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Cytoskeleton-associated protein 5; Colonic and hepatic tumor overexpressed gene protein; Ch-TOG; CKAP5; KIAA0097
Tissue Specificity	Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. Expression is elevated in the brain; highly expressed in the Purkinje cell bodies of the cerebellum.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	Q14008
Gene Id	9793
Gene Name	CKAP5
Subcellular Location	Centrosome. Spindle pole. Spindle. Kinetochore.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, IHC, ICC, WB
Description	Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Human, Mouse, Rat, By Heat<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Human<br> Flow Cytometry (Fixed), 1-3 ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. "Entrez Gene: CKAP5 cytoskeleton associated protein 5".<br /><br />2. Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C (Feb 1996). "Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors". Eur. J. Biochem. 234 (2): 40613.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002214-1	A05324	-	100 ug	$370		Add to Cart
BHA21002214-2	A05324-10ug	Sample	10 ug	$99		Add to Cart
BHA21002214-3	A05324-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002214-4	A05324-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002214-5	A05324-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002214-6	A05324-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002214-7	A05324-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002214-8	A05324-FITC	FITC	100 ug	$570		Add to Cart
BHA21002214-9	A05324-HRP	HRP	100 ug	$570		Add to Cart
BHA21002214-10	A05324-APC	APC	100 ug	$670		Add to Cart
BHA21002214-11	A05324-PE	PE	100 ug	$670		Add to Cart
BHA21002214-12	A05324-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002214-13	A05324-carrier-free	carrier-free	100 ug	$370		Add to Cart

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