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Home / Primary Antibodies / Polyclonal Antibodies

Anti-ONECUT1 Antibody Picoband®

SKU : BHA21005147

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Mfr.No.	A07349, A07349-10ug, A07349-Biotin, A07349-Cy3, A07349-Dylight488, A07349-Dylight550, A07349-Dylight594, A07349-FITC, A07349-HRP, A07349-APC, A07349-PE, A07349-iFluor647, A07349-carrier-free
Alternative Names	mesoderm posterior bHLH transcription factor 1
Background	One cut homeobox 1(ONECUT1), also called Hepatocyte nuclear factor 6(HNF6) is found strong expression in liver and lower expression in testis and skin. ONECUT1 gene is mapped to 15q21.3. This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-ONECUT1 Antibody Picoband® catalog # A07349. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
Host	Rabbit
Immunogen Region	E.coli-derived human ONECUT1 recombinant protein (Position: M1-E270).
Isotype	Rabbit IgG
Molecular Weight	50 kDa
Reactivity	Human
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Synonyms	Mesoderm posterior protein 1; Class C basic helix-loop-helix protein 5; bHLHc5; MESP1; BHLHC5
Tissue Specificity	Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Uniprot	Q9UBC0
Gene Id	3175
Gene Name	ONECUT1
Subcellular Location	Nucleus.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.Avoid repeated freezing and thawing.

Applications	WB, ICC, IF, Flow Cytometry, ELISA
Description	Western blot, 0.25-0.5 ug/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 ug/ml, Human<br> Flow Cytometry (Fixed), 1-3 ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 ug/ml, -<br>

Reference	1. Jacquemin, P., Durviaux, S. M., Jensen, J., Godfraind, C., Gradwohl, G., Guillemot, F., Madsen, O. D., Carmeliet, P., Dewerchin, M., Collen, D., Rousseau, G. G., Lemaigre, F. P. Transcription factor hepatocyte nuclear factor 6 regulates pancreatic endocrine cell differentiation and controls expression of the proendocrine gene ngn3. Molec. Cell. Biol. 20: 4445-4454, 2000.<br /><br />2. Maestro, M. A., Boj, S. F., Luco, R. F., Pierreux, C. E., Cabedo, J., Servitja, J. M., German, M. S., Rousseau, G. G., Lemaigre, F. P., Ferrer, J. Hnf6 and Tcf2 (MODY5) are linked in a gene network operating in a precursor cell domain of the embryonic pancreas. Hum. Molec. Genet. 12: 3307-3314, 2003. <br /><br />3. Samadani, U., Costa, R. H. The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression. Molec. Cell. Biol. 16: 6273-6284, 1996.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21005147-1	A07349	-	100 ug	$370		Add to Cart
BHA21005147-2	A07349-10ug	Sample	10 ug	$99		Add to Cart
BHA21005147-3	A07349-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21005147-4	A07349-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21005147-5	A07349-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21005147-6	A07349-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21005147-7	A07349-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21005147-8	A07349-FITC	FITC	100 ug	$570		Add to Cart
BHA21005147-9	A07349-HRP	HRP	100 ug	$570		Add to Cart
BHA21005147-10	A07349-APC	APC	100 ug	$670		Add to Cart
BHA21005147-11	A07349-PE	PE	100 ug	$670		Add to Cart
BHA21005147-12	A07349-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21005147-13	A07349-carrier-free	carrier-free	100 ug	$370		Add to Cart

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