Porcine Insulin-like Growth Factors Binding Protein 3

Mfr.No.

E0289Po

Description

This sandwich kit is for the accurate quantitative detection of Insulin-like growth factors binding protein 3 (also known as IGFBP-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IBP-3;IGF-binding protein 3;IGFBP 3;IGFBP3;IGFBP-3;Insulin-like growth factor-binding protein 3

Reactivity

Porcine

Detection Range

30-9000ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

31690

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

14.21ng/ml

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Gene ID	448812
UniProt ID	P16611
Antigen	IGFBP3

Research Area

Cell Biology; Signal Transduction; Cardiovascular; Metabolism; Cancer

Protocol

These standard curves of E0289Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/ml	1.868	1.978	1.933
	2.087
2400ng/ml	1	1.034	0.989
	1.067
1200ng/ml	0.405	0.397	0.352
	0.389
600ng/ml	0.31	0.302	0.257
	0.294
300ng/ml	0.088	0.085	0.041
	0.083
0ng/ml	0.032	0.045	0
	0.058

Precision

We measured random samples of E0289Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3193	84.99	2.7
Intra-Assay	2	18	2780.4	95.14	3.4
Intra-Assay	3	18	1468.9	69.39	4.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110691	E0289Po	48T	$320		Add to Cart
BHE12110691	E0289Po	96T	$458		Add to Cart

Porcine Insulin-like Growth Factors Binding Protein 3 - IGFBP3 ELISA Kit

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