Anti-undefined Antibody CTNNB1,2098 Unconjugated BHA10207837

Mfr.No.	1499-MSM11-P0
Alternative Names	Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1
Antigen	Catenin, beta (CTNNB1)
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Cell Surface
Chromosome Location	3p22.1
Clonality	monoclonal
Conjugate	Unconjugated
Description	Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Host	Mouse
Immunogen	Recombinant full-length human beta-Catenin (CTNNB1) protein
Isotype	IgG2b Kappa
Positive Control	HeLa or MCF-7 cells. Breast carcinoma
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P35222
Gene Id	1499
Clone No	CTNNB1, 2098
Symbol	CTNNB1
Weight	92kDa

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IHC-F

Dilution

Immunohistology (Formalin-fixed)

2-4μg/ml for 30 minutes at RT