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Home / Primary Antibodies / Monoclonal Antibodies

HSC70 (HSP73) Antibody, Clone 1F2-H5 - SMC-151

BHA11900720
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+1 866.986.9598
Email
[email protected]
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Swissprot P11142
Accession Number NP_006588.1
Gene Id 3312
Target HSC70 (HSP73)
Clone No 1F2-H5
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~73kDa. Does not cross react with HSP70.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications PBA, AM, ELISA, IHC, PLA, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA ; PLA ; PBA ; AM
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Brown C.L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112.

2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993)J. Mol. Evol. 38(1): 1-17.

3. Rothman J. (1989), Cell 59: 591-601.

4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.

5. Bork P., Sander C., and Valencia A. (1992) Proc. Nut1Acad. Sci. USA 89: 7290-7294.

6. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11900720-1 SMC-151B Unconjugated 200 ug $363
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BHA11900720-2 SMC-151A Unconjugated 50 ug $163
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BHA11900720-3 SMC-151B-A390 ATTO 390 200 ug $465
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BHA11900720-4 SMC-151B-A488 ATTO 488 200 ug $462
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BHA11900720-5 SMC-151B-A594 ATTO 594 200 ug $462
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BHA11900720-6 SMC-151B-ALP Alkaline Phosphatase 200 ug $450
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BHA11900720-7 SMC-151B-APC APC 200 ug $461
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BHA11900720-8 SMC-151B-BI Biotin 200 ug $452
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BHA11900720-9 SMC-151B-FITC FITC 200 ug $444
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BHA11900720-10 SMC-151B-HRP HRP 200 ug $438
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BHA11900720-11 SMC-151B-PCP PerCP 200 ug $461
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BHA11900720-12 SMC-151B-RPE RPE 200 ug $454
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BHA11900720-13 SMC-151S Unconjugated 12 ug $44
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