ELISA: 1/10000, IF/ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Concentration
1 mg/ml
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight
116.7 kDa
Note
This product is for research use only.
Tested Application
ELISA, IHC, FCM
Form
Liquid
Buffer
PBS, containing 0.05% sodium azide
Gene Id
268973
Target
NLR Family CARD Domain Containing 4 Phospho-Ser533 (NLRC4 pS533)Ser533
Purification
Purified from ascites by Protein G chromatography.
Description
NLRC4 (pS533) Antibody is a Mouse Monoclonal against NLRC4 (pS533).
Isotype
IgG
Availability
Shipped within 5-10 working days.
Conjugate
Unconjugated
Reactivity
Human
Host
Mouse
Immunogen
Synthesized peptide corresponding to mouse NLRC4 (Phospho-Ser533) (525-538 AA) expressed in E. coli.
Clonality
monoclonal
Immunofluorescence analysis of HeLa cells using NLRC4 (Phospho-Ser533) Antibody. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555-conjugated phalloidin.
Flow cytometry analysis of HeLa cells using NLRC4 (Phospho-Ser533) Antibody (green) and negative control (red).
Flow cytometry analysis of HeLa cells using NLRC4 (Phospho-Ser533) Antibody (green) and negative control (red).
ELISA analysis. Black line: Control Antigen (100 ng), Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng).
Reviews of NLR Family CARD Domain Containing 4 Phospho-Ser533 (NLRC4 pS533) Antibody