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Human Primary Brachiocephalic Artery Smooth Muscle Cells

SKU : BHC10900075

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T4055
Description	Human Primary Normal Brain Microvascular Endothelial Cells are derived from normal human brain tissue donor. Useful for or cell to cell adhesion assays, or for downstream processes such as RT-PCR or Western blotting for research applications.
Cell Type	Primary Cells
Disclaimer	CD31, VE-Cadherin
Gender	Donor Info Not Disclosed
Growth Properties	Adherent
Quantity	5x105 cells / 1.0 ml
Tissue	Brain
Morphology	Cobble-stone
Species	Human (H. sapiens)
Propagation	The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM104. Cells must be grown on gelatin coated plates. Gelatin Coating Solution (0.1%) is available at abm, TM063. Cells can only propagate for a maximum of 2 passages at split ratio of 1:2. Recommend to conduct media change every 3rd day to maintain optimal growth. Carbon dioxide (CO2): 5%, Temperature: 37.0

Applications	For Research Use Only

Subculture Protocol	1. Remove and discard culture medium.2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.3. Centrifuge cells at 1500rpm for 3 minutes to pellet.4. Aspirate out trypsin, leaving pellet undisturbed.5. Resuspend pellet in fresh culture medium and plate in new culture vessel.6. Incubate cultures at 37°C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10900075	T4055	5x105 cells / 1.0 ml	-		Add to Quote

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