Restriction enzyme digestion of double-stranded DNA
Components
NotI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping
Concentration
20000 U/ml
Heat Inactivation
65°C for 20 min
Quantity
10000 U (500 µl)
Recognition Site
Sequence GC^GGCCGC
Source
An E. coli strain that carries the modified NotI gene from Nocardia otitidis-caviarum (ATCC 1463). The NotI enzyme recognizes and cuts the sequence GC^GGCCGC.
Storage
-20°C
Shipping Condition
Ice Packs
Storage Condition
-20C
Buffer
Storage Buffer: 10 mM Tris-HCl (pH 7.5), 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton® X-100, and 50% (v/v) GlycerolReaction Buffer: Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 25%, Universal Buffer: 100%