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Home / Proteins & Peptide

ExKine Nuclear and Cytoplasmic Protein Extraction Kit

SKU : BHE13700057

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTP3001
Features	Versatilesuitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations. Fast and convenientnon-denatured, active proteins are purified in less than two hours. Compatibleapply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.
Antigen	nuclear
Background	The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in the Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.
Tag	Nuclear
Description	Abbkine ExKine Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue.
Usage	Perform all steps at 28 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Notes	Abbkine ExKine Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.
Kit Components	Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)

Storage	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Description	Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700057	KTP3001	50 T	$139		Add to Cart
BHE13700057	KTP3001	200 T	$299		Add to Cart

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