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Home / Proteins & Peptide

Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active)

SKU : BHP10302793

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSH030690
Description	A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503);without the pro peptide; was fused with a polyhistidine tag at the C-terminus.
Alternative Names	IAP
Reconstitution	Please refer to the printed manual for detailed information.
Background	ALPI encodes for intestinal phosphatase alkaline; a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Reactivity	Human
Tag	C-His
Formulation	Lyophilized from sterile PBS; pH 7.4
Host	HEK293 Cells
Accession Number	P09923
Endotoxin Level	< 1.0 EU per ug of the protein as determined by the LAL method.
Bioactivity	Measured by its ability to cleave a fluorogenic substrate; 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 1;000 pmoles/min/ug.
Mol Weight	54 kDa
Mw(kda)	54 kDa

Sequence Info	Met 1-Asp 503

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 95 % as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10302793	PKSH030690	50ug	$732		Add to Cart

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