A DNA sequence encoding the human AMY2A (P04746) (Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.
Alternative Names
AMY2;AMY2A;PA
Reconstitution
Please refer to the printed manual for detailed information.
Background
Alpha-amylase is the major form of amylase found in humans and other mammals. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.
Reactivity
Human
Tag
C-His
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
P04746
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Mol Weight
57.3 kDa
Mw(kda)
57.3 kDa
Sequence Info
Met 1-Leu511
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.