A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Alternative Names
CAK1;CDKN7;HCAK;MO15;p39MO15;STK1
Reconstitution
Please refer to the printed manual for detailed information.
Reactivity
Human
Tag
N-His
Formulation
Supplied as sterile PBS, pH 7.4
Host
Baculovirus-Insect Cells
Accession Number
P50613&P51946&P51948
Endotoxin Level
< 1.0 EU per ug of the protein as determined by the LAL method.
Bioactivity
The specific activity was determined to be 18 nmol/min/mg using MBP as substrate.
Mol Weight
118.8 (41.2 + 39.7 + 37.9) kDa
Mw(kda)
118.8 (41.2 + 39.7 + 37.9) kDa
Sequence Info
Ala 2-Phe 346&Tyr 2-Leu 323&Asp 2-Ser 309
Shipping
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stotage Note
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.