A DNA sequence encoding human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed with a linker AAK.
Alternative Names
IDDM;IDDM1;IDDM2;ILPR;INS;IRDN;MODY10
Reconstitution
Please refer to the printed manual for detailed information.
Background
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Reactivity
Human
Formulation
Lyophilized from sterile PBS, pH 7.4.
Host
Yeast
Accession Number
P01308
Endotoxin Level
Please contact us for more information.
Bioactivity
Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells.The ED50 for this effect is typically 0.2-1 µg/mL.
Mol Weight
6 kDa
Mw(kda)
6 kDa
Sequence Info
Phe1-Lys29&Gly1-Asn21
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.