Please refer to the printed manual for detailed information.
Background
Nicotinamide phosphoribosyltransferase (NAMPT); also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin; is an enzyme belonging to the family of glycosyltransferases; to be specific; the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1- pyrophosphate to yield nicotinamide mononucleotide; one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis in healthy individuals and in individuals with severe congenital neutropenia. Intracellular NAMPT and NAD+ amounts in myeloid cells; as well as plasma NAMPT and NAD+ levels; were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.
Reactivity
Human
Tag
N-His & GST
Formulation
Lyophilized from sterile 20mM Tris; 500mM NaCl; pH 8.0; 20% gly; 0.3mM DTT 1. Normally 5 % - 8 % trehalose; mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements.
Host
Baculovirus-Insect Cells
Accession Number
P43490
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Mol Weight
83.3 kDa
Mw(kda)
83.3 kDa
Sequence Info
Met 1-His 491
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.