A DNA sequence encoding the rat ASAM (Q8K1G0) (Met1-Met232) was expressed, fused with a polyhistidine tag at the C-terminus.
Alternative Names
ASAM;Clmp ,Acam
Reconstitution
Please refer to the printed manual for detailed information.
Background
Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.
Reactivity
Rat
Tag
C-His
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
Q8K1G0
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 10E4 cells/well are added to Recombinant Rat ASAM coated plates (12.5 ug/mL with 100 uL/well), 35-70% cells will adhere after 1 hour incubation at 37?.
Mol Weight
25.6 kDa
Mw(kda)
25.6 kDa
Sequence Info
Met1-Met232
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.