A DNA sequence encoding the Zika virus (ZIKV) (strain ZikaSPH2015) NS5 (ALU33341.1) (Lys2772-Leu3423) was expressed with a polyhistidine tag at the C-terminus.
Alternative Names
Zika virus, NS5 protein, strain Zika SPH2015
Reconstitution
Please refer to the printed manual for detailed information.
Background
Zika virus NS5 is invloved in methytransferase and RNA guanylytransferase activities and capping and synthesis of RNA. And, NS5 is also a RNA-dependent RNA polymerase.
Reactivity
ZIKV
Tag
C-His
Formulation
Lyophilized from sterile 20 mM PB,100 mM NaCl,50% glycerol, pH 7.0, 0.5 mM TCEP.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Host
Baculovirus-Insect Cells
Accession Number
ALU33341.1
Endotoxin Level
< 1.0 EU per ?g protein as determined by the LAL method.
Mol Weight
76.7kDa.
Mw(kda)
76.7kDa.
Sequence Info
Lys2772-Leu3423
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with ice bag. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.