Matrices listed below were spiked with certain level of Jag1 and the recovery rates were calculated by comparing the measured value to the expected amount of Jag1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
95
EDTA plasma(n=5)
97-102
100
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Jag1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Jag1 ELISA Kit allows for the in vitro quantitative determination of Jag1 concentrations in serum, plasma, tissue homogenates and other biological fluids.