Rat Latent Transforming Growth Factor Beta Binding Protein 1

Mfr.No.

E0944Ra

Alternative Names

Latent-transforming growth factor beta-binding protein 1;LTBP 1;LTBP1;LTBP-1;TGF-beta-1-BP-1;Transforming growth factor beta-1-binding protein 1;Transforming growth factor beta-1-masking protein large subunit

Reactivity

Rat

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

186599

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.14ng/L

Background

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed: 7593177). Associates specifically via disulfide bonds with the Latency-associated Peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (By similarity). Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta (By similarity).

Gene ID	59107
UniProt ID	Q00918
Antigen	LTBP1

Research Area

Signal Transduction

Protocol

These standard curves of E0944Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.651	2.575	2.531
	2.499
1200ng/L	1.457	1.505	1.46
	1.552
600ng/L	0.712	0.778	0.734
	0.845
300ng/L	0.382	0.417	0.372
	0.451
150ng/L	0.211	0.223	0.179
	0.235
0ng/L	0.039	0.044	0
	0.049

Precision

We measured random samples of E0944Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	970.1	46.33	4.8
Intra-Assay	2	18	1237.1	42.39	3.4
Intra-Assay	3	18	250.9	12.47	5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112193	E0944Ra	48T	$320		Add to Cart
BHE12112193	E0944Ra	96T	$458		Add to Cart

Rat Latent Transforming Growth Factor Beta Binding Protein 1 - LTBP1 ELISA Kit

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