Matrices listed below were spiked with certain level of RTEL1 and the recovery rates were calculated by comparing the measured value to the expected amount of RTEL1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
86-103
94
heparin plasma(n=5)
85-98
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RTEL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-97%
91-105%
87-101%
EDTA plasma(n=5)
83-97%
85-96%
85-101%
heparin plasma(n=5)
85-94%
80-98%
82-96%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q5RJZ1
Antigen
RTEL1
Applications
ELISA
Applications Notes
RTEL1 ELISA Kit allows for the in vitro quantitative determination of RTEL1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat RTEL1(Regulator of telomere elongation helicase 1) ELISA Kit