Anti-undefined Antibody CALD1,1424R Unconjugated BHA10207829

Mfr.No.	800-RBM4-P0
Alternative Names	CAD ; CALD1 ; Caldesmon 1 Isoform 1 ; Caldesmon 1 Isoform 2 ; Caldesmon 1 Isoform 3 ; Caldesmon 1 Isoform 4 ; Caldesmon 1 Isoform 5 ; CDM ; HCAD ; LCAD ; NAG22
Antigen	Caldesmon, HMW (h-Caldesmon)
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	7q33
Clonality	Monoclonal
Conjugate	Unconjugated
Description	Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Host	Rabbit
Immunogen	Recombinant full-length human CALD1 protein
Isotype	IgG
Positive Control	Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Reactivity	Human, Rat
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	Q05682
Gene Id	800
Clone No	CALD1, 1424R
Symbol	CALD1
Weight	150kDa

Buffer	200μg/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

FACS, IF, IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution