We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Tag
C-terminal 10xHis-tagged
Molecular Weight
45.3 kDa
Relevance
Isoform 1<br />Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner.<br />Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis.<br />Isoform 8<br />Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner.<br />Promotes populations of T cells regulating IgA production and secretion associated with control of the commensal microbiota and resistance to enteropathogens.
Source
Yeast
Form
Lyophilized powder
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Purity
Greater than 95% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. <br />Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reference
Cell adhesion molecule CEACAM1 associates with paxillin in granulocytes and epithelial and endothelial cells.<br />Ebrahimnejad A., Flayeh R., Unteregger G., Wagener C., Bruemmer J.<br />Exp. Cell Res. 260:365-373 (2000)