We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Tag
N-terminal 6xHis-KSI-tagged
Molecular Weight
20.5 kDa
Relevance
LEA protein are late embryogenesis abundant in higher plant seed embryos. There are two subsets of LEA proteins (5a, and 5b), the first ones are expressed when the cotyledon weight reach 80 mg and the second set are expressed above 100 mg. The function of those proteins is not known.
Source
E.coli
Endotoxin Level
Not test.
Form
Liquid or Lyophilized powder
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Bioactivity
Not Test
Expression Region
1-48aa
Lead Time
13-23 business days
Organism
Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus)
Protein Length
Partial
Uniprot
P21298
Research Area
Others
Sequence
MADLKDERGNPIHLTDAYGNPVQLSDEFGNPMHITGVASSAPQYKDSV
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Purity
Greater than 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reviews of Recombinant Raphanus sativus Late embryogenesis abundant protein, partial