Firefly luciferase facilitates the conversion of luciferin to oxyluciferin in conditions where ATP, magnesium ions, and oxygen are present, resulting in the emission of bioluminescence at a wavelength of about 560 nm, detectable with a luminometer. CleanRNA Firefly Luciferase-mRNA (low dsRNA) is an mRNA is an mRNA that has been modified with a 5' cap1, a 3' poly(A) tail, and N1-Methyl-Pseudouridine Triphosphate (N1-Me-Pseudo UTP). The product is created through an in vitro transcription process using CleaScripT7 RNA polymerase, which generates ultralowdsRNA content in the mRNA solution, thereby improving the in vivo performanceof the Firefly Luciferase-mRNA.
Specifications
Catalog Number
N120011S/N120011M
Specifications
0.5 mg/1 mg
mRNA length
2078 nt
Concentration
1 mg/mL
Capping efficiency
100%
dsRNA
?0.1 ng/?g
Storage buffer
1 mM sodium citrate
Components
Component
Number
Component Name
Storage
Temperature
N120011S
N120011M
N120011
CleanRNA Firefly Luciferase-mRNA (low dsRNA)
-80°C
0.5 mg
1 mg
Figure
Integrity tested by CE (capillary electrophoresis)
Capping efficiency tested by MS
Shipping and Storage
Storage
The products are shipped with dry ice and can be stored at -80? for two year.