Sheep Corticoliberin - CRH ELISA Kit

Mfr.No.

E0162Sh

Alternative Names

Corticoliberin;Corticotropin-releasing factor;Corticotropin-releasing hormone;CRF;CRH;Endorpholiberin

Reactivity

Sheep

Detection Range

3-900ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

20672

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.54ng/L

Background

Hormone regulating the release of corticotropin from pituitary gland (PubMed:6267699, PubMed:6273874). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).

Gene ID	101121525
UniProt ID	P01142
Antigen	CRH

Research Area

Signal Transduction

Protocol

These standard curves of E0162Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.27	2.29	2.254
	2.31
240ng/L	1.393	1.379	1.344
	1.366
120ng/L	0.782	0.752	0.716
	0.721
60ng/L	0.292	0.37	0.334
	0.447
30ng/L	0.124	0.141	0.105
	0.158
0ng/L	0.026	0.036	0
	0.045

Precision

We measured random samples of E0162Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	172.13	7.46	4.33
Intra-Assay	2	18	334.12	15.4	4.61
Intra-Assay	3	18	72.79	2.59	3.56

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113630	E0162Sh	48T	$320		Add to Cart
BHE12113630	E0162Sh	96T	$458		Add to Cart

Sheep Corticoliberin - CRH ELISA Kit

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