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Home / Biochemicals / Small Molecules

1-Norokadaone

SKU : BHB21300250

An Inactive Homologue of Okadaic Acid and Okadaol, Inhibitors of Protein Phosphatase 1 and Protein Phosphatase 2A

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Mfr.No.	N-750, N-750_5 mcg
Description	1-Norokadaone (#N-750) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C43H66O11.
Background	1-Norokadaone is structural analogue of Okadaic acid. This compound is a less active homologue than the Okadaic Acid and Okadaol as inhibitors of Protein Phosphatase 1 and Protein Phosphatase 2A, and is thus widely used as negative control for Okadaic Acid1-3.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Cas Number	131204-29-0
Molecular Weight	758.99
Solubility	DMSO, ethanol, acetone or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
Target	Protein Phosphatase 1 and Protein Phosphatase 2A
Source	Synthetic
Activity	1-Norokadaone is an inactive analogue of okadaic acid (a PP1 and PP2A inhibitor) even at high concentrations1. It may be used as a negative control vis-A -vis okadaic acid.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: 1-(11-Hydroxy-2-((E)-4-(4-hydroxy-2-(1-hydroxy-3-(3- methyl-1,7-dioxaspiro(5.5)undecan-2-yl)butyl)-3-methylidenespiro (4a,7,8,8a-tetrahydro-4H-pyrano(3,2-b)pyran-6,5'-oxolane)-2'-yl)but-3-en-2-yl)-4-methyl-1,7-dioxaspiro(5.5)undec-4-en-8-yl)propan-2-one.

Purity	>98%
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to one week at 4°C or three months at -20°C.Protect from light.

Product Manual	SDS

Reference	Bastan, R. et al. (2001) Eur J Pharmacol, 430, 135. Honkanen, R.E. et al. (1994) Toxicon 32, 339. Nishiwaki, S. et al. (1990) Carcinogenesis 11, 1837. Gurland, G. and Gundersen, G.G. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 8827.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300250-1	N-750_5 mcg	5 mcg	$261		Add to Cart

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