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Home / Biochemicals / Small Molecules

1-Phenylbiguanide hydrochloride

SKU : BHB21300267

A Potent and Selective Agonist of 5-HT3 Receptors

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Mfr.No.	P-190, P-190_1 g, P-190_5 g
Description	1-Phenylbiguanide hydrochloride (#P-190) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C8H12ClN5.
Synonyms	Synthetic
Background	Phenylbiguanide (PBG) acts as an agonist of 5-HT3 receptors, with an EC50 value two fold greater than that of 5-HT and was found to imitate the effects of 5-HT on mammalian peripheral neurons. PBG causes dopamine release and has been used in many studies as a selective agonist to arouse reflex bradycardia and hypotension through activation of cardiac and pulmonary vagal afferents1,3.For over 40 years, PBG has been used as a selective agonist to identify cardiac and pulmonary vagal C fibers and the associated reflex bradycardia and depressor responses. It is capable to stimulate cardiac chemosensitive receptors with vagal afferent pathways and the sensory nerves in different regions of the body3.In one study conducted in rats, it was found that phenylbiguanide depolarizes the rat vagus nerve and mimics the effects of 5-HT on the vagus nerve. In addition, it was shown that phenylbiguanide causes reflex falls in heart rate and blood pressure in the cat and induces pain following application to the bases of blisters in human subjects1.It has been documented that right atrial bolus injection of phenylbiguanide can stimulate bronchopulmonary C-fibers that play an important role in modulating the respiratory rhythm to produce apnea2.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	0.1-100 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Cas Number	55-57-2
Molecular Weight	213.67
Solubility	25 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Target	Serotonin-gated channels
Activity	1-Phenylbiguanide hydrochloride is an activator of 5-HT3 channels1.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: 1-(diaminomethylidene)-2-phenylguanidine;hydrochloride.

Purity	>99%
Form	Lyophilized powder.
Storage	Storage of solutions: Up to four weeks at 4°C or three months at -20°C.

Product Manual	SDS

Reference	Ireland, S.J. and Tyers, M.B. (1987) Br. J. Pharmacol. 90, 229. Zhang, Z. et al. (2013) Neurosci. Lett. 552, 10. Fu, L.W. and Longhurst, J.C. (1998) Am. J. Physiol. 275, 6. Ireland, S.J. and Tyers, M.B. (1987) Br. J. Pharmacol. 90, 229.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300267-1	P-190_1 g	1 g	$31		Add to Cart
BHB21300267-2	P-190_5 g	5 g	$50		Add to Cart

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