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Home / Biochemicals / Small Molecules

NS-309

SKU : BHB21300233

A Positive Modulator of Small- and Intermediate-Conductance KCa Channels

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Mfr.No.	N-180, N-180_10 mg, N-180_25 mg, N-180_5 mg, N-180_50 mg
Description	NS-309 (#N-180) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C8H4Cl2N2O2.
Background	Ca2+-activated potassium channels (KCa) are a group of 6/7-TM proteins that selectively transport K+ ions across biological membranes. They are broadly classified into three subtypes: SK, IK and BK channels (small, intermediate and big conductance, respectively). Three SK channel subtypes exist (SK1-3, KCa2.1-3)1 and are widely distributed in the central nervous system (CNS)1-2 as well as in the periphery. They constitute a molecular family of voltage-independent channels with the peripherally expressed intermediate conductance Ca2+-activated K+ channel (IK, KCa3.1)3, but differ in their distribution; SK channels are predominantly expressed in excitable cells, whereas IK channels mostly are found in non-excitable cells3-4.NS-309 is a selective and potent activator of KCa3.1 and KCa2 channels, whereas it is devoid of effect on BK (KCa1.1) type channels5-6. Whole cell currents, obtained by voltage ramps applied to HEK293 cells, reveal that human KCa2 channels are two to four times less sensitive to NS-309 than human KCa3.1; NS-309 increases the KCa2 currents with an EC50 value of 30 nM, whereas an EC50 value of 10 nM is inferred for the KCa3.1 currents5.NS-309 possesses a therapeutic potency for cystic fibrosis and chronic obstructive pulmonary disease by improving epithelial Cl- secretion via KCa3.1 channel activation7 and for moderating neuronal hyperexcitability by enhancing apamin-sensitive after-hyperpolarizing current (IAHP, mediated by KCa2 channels)5-6.NS-309 shows substantial block of L-type Ca2+ channel. It concentration-dependently inhibits the ICa in smooth muscle cells isolated from mouse urinary bladder with an IC50 value of 10.6 mM8. However, the specificity of NS-309 for the KCa3.1 channel over VDCC is much higher.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	0.1 - 10 uM.
Reconstitution	DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Cas Number	18711-16-5
Molecular Weight	231.04
Solubility	DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Target	KCa2 and KCa3.1 K+ channels
Source	Synthetic
Activity	NS-309 is a positive modulator of small- and intermediate- conductance Ca2+-activated K+ (KCa2 and KCa3.1) channels, with EC50 values of 30 and 10 nM, respectively1. NS-309 also blocks L-type Ca2+ channels with IC50 value of 10 uM2.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: 6,7-Dichloro-1H-indole-2,3-dione 3-oxime.

Purity	>99% (HPLC)
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to four weeks at 4°C or three months at -20°C.

Product Manual	SDS

Reference	Kohler, M. et al. (1996) Science 273, 1709. Stocker, M. et al. (2000) Mol Cell Neurosci. 15, 476. Ishii, T.M. et al. (1997) Proc. Natl. Acad. Sci. U.S.A 94, 11651. Jensen, B.S. et al. (1998) Am. J. Physiol. 275, C848. Strøbaek, D. et al. (2004) Biochim Biophys. Acta. 1665, 1. Pedarzani, P. et al. (2005) J. Biol. Chem. 280, 41404. Singh, S. et al. (2001) J. Pharmacol. Exp. Ther. 296, 600. Morimura, K. et al. (2006) J. Pharmacol. Sci. 100, 237. Strøbaek, D. et al. (2004) Biochim Biophys. Acta. 1665, 1. Morimura, K. et al. (2006) J. Pharmacol. Sci. 100, 237.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300233-1	N-180_10 mg	10 mg	$75		Add to Cart
BHB21300233-2	N-180_25 mg	25 mg	$129		Add to Cart
BHB21300233-3	N-180_5 mg	5 mg	$55		Add to Cart
BHB21300233-4	N-180_50 mg	50 mg	$223		Add to Cart

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