JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Biochemicals / Small Molecules

Ro46-8443

SKU : BHB21300309

A Selective Antagonist of Endothelin B Receptors

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Alomone Labs

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	R-185, R-185_1 mg, R-185_10 mg, R-185_25 mg, R-185_5 mg
Description	Ro46-8443 (#R-185) is a highly pure, synthetic, and biologically active compound.
Molecular Formula	C31H35N3O8S.
Background	Ro46-8443 is a synthetic compound that acts as a selective and competitive antagonist of the ET-B (endothelin B) receptor, a receptor that belongs to the G-protein coupled receptor (GPCR) superfamily. Ro46-8443 shows a 100-fold selectivity for human ET-B over ET-A receptors and is being used as an important tool for studying the role of ET-B receptors in pathology1. Ro 46-8443 demonstrates IC50 values of 34 nM and 69 nM in competitive binding assays for human ET-B receptors in radiolabeled membranes of Chinese hamster ovary cells and microsomal membranes from human placenta, respectively. The compound also inhibits the binding of selective radiolabeled ET-B agonist Sarafotoxin-S6c to porcine trachea membranes with an IC50 value of 4.9 nM1.Endothelin receptors are widely distributed in vascular and nonvascular tissues. The ET-B receptor has high and equal affinity for all endothelin isopeptides3. ET-B receptors play an important role in regulating renal function and blood pressure and are expressed in sensory nerves. They are mainly present in medium- and large-sized cell bodies of human trigeminal ganglia3.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	0.1-10 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Cas Number	175556-12-4
Molecular Weight	609.69
Solubility	Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).
Target	ET-B receptor
Source	Synthetic
Activity	Ro 46-8443 is a non-peptide, selective and competitive antagonist of ET-B receptors. It shows over 100-fold selectivity for human ET-B in competitive binding assays (IC50 of 34-69 nM), and competitively inhibits the binding of selective ET-B agonist (125I)Sarafotoxin-S6c to porcine trachea membranes (IC50 = 4.9 nM)1.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Chemical Name: 4-tert-butyl-N-(6-((2R)-2,3-dihydroxypropoxy)-5-(2-methoxyphenoxy)-2-(4-methoxyphenyl)pyrimidin-4-yl)benzenesulfonamide.

Purity	>95%
Form	Lyophilized powder.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to three months at -20°C.

Product Manual	SDS

Reference	Breu, V. et al. (1996) FEBS Lett. 383, 37. Saeki, T. et al. (1991) Biochem. Biophys. Res. Commun. 179, 286. Xie, C. and Wang, D.H. (2009) Hypertension 54, 1298. Breu, V. et al. (1996) FEBS Lett. 383, 37.

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300309-1	R-185_1 mg	1 mg	$117		Add to Cart
BHB21300309-2	R-185_10 mg	10 mg	$645		Add to Cart
BHB21300309-3	R-185_25 mg	25 mg	$1106		Add to Cart
BHB21300309-4	R-185_5 mg	5 mg	$406		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2025 Biohippo. All rights reserved.