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Home / Biochemicals / Small Molecules

Ryanodine

SKU : BHB21300315

A Blocker of Ryanodine Receptors

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Mfr.No.	R-500, R-500_0.5 mg, R-500_1 mg, R-500_5 mg
Description	Ryanodine (#R-500) is a highly pure, natural, and biologically active compound.
Molecular Formula	C25H35NO9.
Background	Ryanodine is found naturally in the stem and roots of the plant Ryania speciosa. The ryanodine alkaloid was first isolated as a potential insecticide1 but was found to act as a potent paralytic on skeletal and cardiac muscles.2 Ryanodine inhibits SR Ca2+ release by binding with high affinity to the ryanodine receptor (RyR) channel as a tetrameric complex.3,4The RyR channel has three isoforms and is a poorly selective Ca2+ channel (selectivity Ca2+/K+ ~6) with very high conductance (~700 pS with K+ as charge carrier and ~100 pS with Ca2+ as charge carrier). The channel is regulated by Ca2+, Mg2+, ATP, and caffeine. Application of ryanodine locks the channel in a slow-gating subconductance state. Opening of the ryanodine-bound channel is long-lived and gives rise to a smaller unit current (~1/3 or ~1/2) than the control amplitude. This action of ryanodine on single RyR channel function is quite distinctive and is often used for functional identification of the channel.5-8Ryanodine binds with high affinity to RyR proteins in a pore located in the carboxyl terminus of the protein.9 Binding induces a complex change in single RyR channel function. This change is similar in all three RyR channel isoforms, inducing open events of very long duration and simultaneous reduction of ion conductance through the pore.10,7 This dual impact on single-channel function (i.e. slower gating and reduced conductance) suggests a complicated mode of action. This is also supported by the rather complicated ryanodine dose dependency. Low doses of ryanodine (~10 nM) are reported to increase the frequency of single RyR channel openings to the normal conductance level. Intermediate ryanodine doses (~1 uM) are reported to induce the classical ryanodine action described above. High doses of ryanodine (~100 uM) are reported to lock the channel in a closed configuration.11,10It is generally assumed that each RyR monomer contains one high-affinity ryanodine binding site (KD
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	1-25 uM.
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Cas Number	15662-33-6
Molecular Weight	493.5
Solubility	Methanol. Centrifuge all product preparations before use (10000 x g 5 min).
Target	Ryanodine receptors
Source	Natural
Activity	Ryanodine is a blocker of the Ryanodine receptor (RyR) Ca2+ release channel1.
Country Of Origin	Israel/IL
UNSPSC Code	41116134
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Bioassay Tested: Yes Cited Application: Electrophysiology Chemical Name: 1H-Pyrrole-2-carboxylic acid,(3S,4R,4aR,6S,7S,8R,8aS,8bR,9S,9aS)-dodecahydro-4,6,7,8 a,8b,9a-hexahydroxy-3,6a,9-trimethyl-7-(1-methylethyl)- 6,9-methanobenzo(1,2)pentaleno(1,6-bc)furan-8-yl ester.

Purity	>98%
Form	Lyophilized Powder
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to one week at 4°C or six months at -20°C.

Product Manual	SDS

Reference	Elison, C. and Jenden, D.J. (1967) Biochem. Pharmacol. 16, 1347. Frank, M. and Sleaton, W.W. (1975) Res. Commun. Chem. Pathol. Pharmacol. 11, 65. Campbell, K.P. et al. (1987) J. Biol. Chem. 262, 6460. Lai, F.A. et al. (1989) J. Biol. Chem. 264, 16776. Hymel, L. et al. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 441. Lai, F.A. et al. (1988) Nature 331, 315. Smith, J.S. et al. (1988) J. Gen. Physiol. 92, 1. Tu, Q. et al. (1994) Biophys. J. 67, 2280. Ogawa, Y. (1994) Crit. Rev. Biochem. Mol. Biol. 29, 229. Rousseau, E. et al. (1987) Am. J. Physiol. Cell. Physiol. 253, C364. Buck, E. et al. (1992) J. Biol. Chem. 267, 23560. Ogawa, Y. (1994) Crit. Rev. Biochem. Mol. Biol. 29, 229. Chu, A. et al. (1990) Mol. Pharmacol. 37, 735. Sutko, J.L. et al. (1997) Pharmacol. Rev. 49, 53.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300315-1	R-500_0.5 mg	0.5 mg	$295		Add to Cart
BHB21300315-2	R-500_1 mg	1 mg	$400		Add to Cart
BHB21300315-3	R-500_5 mg	5 mg	$1008		Add to Cart
BHB21300315-4	R-500_1 mg	5x1 mg	$992		Add to Cart

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