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Home / Biochemicals / Small Molecules

Tetrodotoxin citrate

SKU : BHB21300354

Tetrodotoxin citrate (#T-550) is a highly pure, natural, and biologically active compound.

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Mfr.No.	T-550, T-550_1 mg, T-550_50 mcg
Description	A Classic Blocker of a Subset of NaV Channels
Molecular Formula	C11H17N3O8
Synonyms	Natural
Background	Tetrodotoxin (TTX) is an established potent and selective blocker of some NaV channels.1 Moreover, TTX sensitivity is used to define subclasses of NaV channels.2 It is widely used both to probe NaV activity and to reduce or block excitability in neurophysiological preparations.
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Concentration	100 nM - 1 uM
Reconstitution	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. TX citrate is freely soluble in water to over 10 mM The reconstituted product contains citrate buffer.To reconstitute 1 mg lyophilized product to 10 mM stock solution - add 313 ul ddH2O to the vial and tap gently until fully dissolved. To reconstitute 50 ug lyophilized product to 1 mM stock solution - add 157 ul ddH2O to the vial and tap gently until fully dissolved.Centrifuge all product vials before handling and reconstitution (10000 x g 5 min).
Cas Number	18660-81-6
Molecular Weight	319.27
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. TX citrate is freely soluble in water to over 10 mM The reconstituted product contains citrate buffer.To reconstitute 1 mg lyophilized product to 10 mM stock solution - add 313 ul ddH2O to the vial and tap gently until fully dissolved. To reconstitute 50 ug lyophilized product to 1 mM stock solution - add 157 ul ddH2O to the vial and tap gently until fully dissolved.Centrifuge all product vials before handling and reconstitution (10000 x g 5 min).
Target	Various NaV channels
Activity	Tetrodotoxin is a potent and selective blocker of a subclass of NaV channels1, and is often used to define subclasses of NaV channels2.
Country Of Origin	Israel/IL
UNSPSC Code	12352202
Application	FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay
Lead Time	1-2 Business Days
Notes	Is Toxin: Yes Bioassay Tested: Yes Chemical Name: Octahydro-12-(hydroxymethyl)-2-imino-5,9:7,10a-dimethan o-10aH-(1,3)dioxocino(6,5-d)pyrimidine-4,7,10,11,12-pentol.

Purity	>99% (HPLC)
Form	Lyophilized
Storage	Storage of solutions: Up to one week at 4°C or six months at -20°C.

Product Manual	SDS

Reference	Narahashi, T. et al. (1964) J. Gen. Physiol. 47, 965. Hille, B. (2001) In: Ion Channels in Excitable Membranes (third edition) chapter 3. Narahashi, T. et al. (1964) J. Gen. Physiol. 47, 965. Hille, B. (2001) Ion Channels in Excitable Membranes (Third Edition) Chapter 3.

Applications	Electrophysiology

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHB21300354-1	T-550_1 mg	1 mg	$430		Add to Cart
BHB21300354-2	T-550_50 mcg	50 mcg	$173		Add to Cart
BHB21300354-3	T-550_1 mg	5x1 mg	$1719		Add to Cart

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