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Synthetic DNA Libraries

Online Inquiry

Synbio Technologies is equipped with the world leading Syno® DNA technology platforms ranged from standard gene synthesis to the proprietary next generation Syno® 3.0 synthesis technology. We are capable of generating high quality DNA libraries of any size and complexity with an efficient timeframe and lower cost. Our synthetic DNA libraries can facilitate your various research interests: spanning from functional genetics to directed evolution, especially to protein engineering and antibody studies. In addition, Synbio Technologies’ experienced bioinformatics team can help to analyze and provide synthetic DNA libraries design to your researches.

Competitive Advantages

  • Generation of Multiple Library Types: Scanning, degenerate codon, triplet codon, site saturation, random substitution, modular etc.
  • Professional Technical Platforms: The flexible Syno® 2.0 and 3.0 synthesis platforms guarantee
  • a rapid and high throughput gene synthesis providing a powerful ability to successfully assemble large DNA fragments with 100% sequence accuracy.
  • Flexible Deliverables: Oligo pools, PCR fragments, pooled clones or error-free separated colonies.
  • Achievable Library Diversity: We can offer the library diversity of up to 1014 variants.

Applications of Synthetic DNA Libraries

  • Alanine Scan Library : Research on amino acid residues critical to protein function, interaction and shape by replacing an individual amino acid, with alanine, at every position.
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  • Site Saturation Library : Substitute all 20 possible amino acids at any site to evaluate structure-function relationships and to generate improved protein variants.
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  • Random Substitution Library : Substitute bases with triplet, random or degenerate ones to identify locations for specific analysis.
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  • Antibody Library : Create a high diversity synthetic antibody library or improve existing antibody functionality such as specificity, immunogenicity, affinity, expression and/or aggregation.
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  • Modular Substitution Library : Build new genetic modules, regions or pathways from DNA parts including regulatory elements and/or coding sequences.
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Deliverables

Pooled ss Oligo Libraries Pooled ds PCR Libraries Pooled Cloning Libraries Error Free Separated Colonies Libraries
Length Up to 125nt/200nmol Up to 3kb Up to 109 transformants Up to 1M colonies
Fidelity Oligo mix with degenerated base(s) or triplet base(s) PCR fragment pool with variants Pool of variant sequences cloned into vector 100% sequence verified clones in a vector
Complexity high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes 30-80% GC, no repeats high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes high or low GC regions, GC flux, tandem repeats, homopolymers, palindromes
Custom Vector – – Available Available
Quality Assurance Amplification, Cloning and sequencing at least 16-96 clones or NGS to verify diversity Cloning and sequencing at least 16-96 clones or NGS to verify diversity Sequencing at least 16-96 clones or NGS to verify diversity 100% sequence verification using Next Generation and Sanger sequencing methods

Pricing and Delivery Times

Length (nt/kb) Complexity Analysis Diversity Analysis by Sequencing Oligo or PCR fragment Yield Pooled Cloning Plasmids/Culture Yield Error-free Separated Plasmids Yield Pooled ss Oligo Libraries Pooled DNA Library Priced Clone Library List Price Delivery Time*
0.02-0.125 √ √ 100 nmol – – Inquire – – 10-15 Business Days
0.125-3.0 √ √ 2 μg 20-100 μg/1-5ml culture 1-5 μg – $0.15/bp plus degerated/triplet price $0.35/bp plus degerated/triplet price 10-15 Business Days
1.5-3.0 √ √ 2 μg 20-100 μg/1-5ml culture 1-5 μg – $0.20/bp plus degerated/triplet price $0.45/bp plus degerated/triplet price 10-15 Business Days
3.0-6.0 √ √ – 20-100 μg/1-5ml culture 1-5 μg – – $0.55/bp plus degerated/triplet price 20-40 Business Days
6.0-9.0 √

√

 – 20-100 μg/1-5ml culture 1-5 μg – – $0.65/bp plus degerated/triplet price 30-50 Business Days
>9.0 √ √ – 20-100 μg/1-5ml culture 1-5 μg – – $0.95/bp plus degerated/triplet price Inquire

Related Services

If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.

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