Hieff Trans™ Booster DNA/RNA Transfection Reagent

Mfr.No.

40801ES

Description

Booster DNA/RNA Transfection Reagent is an advanced nucleic acid transfection reagent developed based on novel polymeric materials. Its unique molecular design significantly reduces cytotoxicity and features a smart anti-enzymatic degradation mechanism that protects nucleic acids from intracellular enzymatic degradation during delivery, thereby maximizing transfection efficiency. This reagent is suitable for various types of nucleic acids including DNA, siRNA, miRNA, mRNA, and ASO, and has been validated for efficient transfection in cell lines such as 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW267.4, HCT116, as well as primary cells, providing reliable tools for gene research.

Componentsrnrn

Components No.	Name	40801ES01	40801ES04
40801-A	Booster DNA/RNA Transfection Reagent	100 μL	1.5 mL
40801-B	Transfection Enhancer	100 μL	1.5 mL

Application
Cell Transfection; DNA Transfection; mRNA/SiRNA Transfection;

Features

Low Cytotoxicity: The novel polymer replaces traditional liposomes to reduce cytotoxicity and improve cell viability.

Fast Release and Higher Efficiency: Fast release minimizes intracellular nucleic acid degradation, achieving >90% transfection efficiency (measured by flow cytometry).

Premium Options for Difficult-to-transfect cell lines: Sensitive and primary cells.

Proven Performance, Broad Cell Line Coverage: Achieves high efficiency in 200+ various cell lines including 293T, HeLa, MCF7, HepG2, A549, NIH3T3, RAW264.7, and HCT116 and primary cells, with consistent and reproducible results.

One Reagent, All Nucleic Acids: Delivers DNA, siRNA, miRNA, mRNA, and ASO with high efficiency—even up to 15 kb fragments.

Storage	This product should be stored at 2~8℃ for 12 months.

Other Protocols	Manual Safety Data Sheet
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High-Efficiency Primary Cell BMDM Transfection. Plasmid DNA transfection in primary bone marrow-derived macrophages (BMDMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

High-Efficiency DNA Transfection. DNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior DNA transfection efficiency of Booster.

High-Efficiency mRNA Transfection. mRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior mRNA transfection efficiency of Booster.

High-Efficiency siRNA Transfection. siRNA transfection across different cells using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior siRNA transfection efficiency of Booster.

High-Efficiency Primary Dermal Fibroblasts Transfection. Plasmid EGFR DNA transfection in primary dermal fibroblasts using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

High-Efficiency Porcine Alveolar Macrophages Transfection. DNA transfection in primary Porcine Alveolar Macrophages (PAMs) using Booster DNA&RNA Transfection Reagent versus L*3000. The result demonstrates superior primary cell transfection efficiency of Booster.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHM20800021-1	40801ES01	100 μL	$75		Add to Cart
BHM20800021-2	40801ES04	1.5 mL	$645		Add to Cart

Hieff Trans™ Booster DNA/RNA Transfection Reagent _ 40801ES

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