Hieff Trans™ UltraAAV Transfection Reagent (PEI, GMP grade)

Mfr.No.

40824ES, 40824ES10

Description

This product is specifically developed for the large-scale production of AAV in suspension systems. It is an optimized and modified linear polyethylenimine (Polyethylenimine Linear, PEI). The product is synthesized purely through chemical processes, free from animal-derived components, and has low cytotoxicity. It also offers broad applicability across various AAV serotypes. With high DNA loading capacity, a low transfection complex ratio, and high complex stability, this product facilitates large-scale AAV production.

The product is manufactured in accordance with GMP requirements and is provided in the form of a sterile liquid.

Applications
Production of AAV Viral Vectors

Specificationsrnrnrnrn

Form	liquid
Properties	Polyethylenimine Linear (PEI)
Virus Type	AAV

Componentsrnrn

Components No.	Name	40824ES10	40824ES60	40824ES80
40824	Hieff Trans™ PEI AAV Transfection Reagent-GMP	10 mL	100 mL	1 L

Features

Significant Increase in Virus Titer: The product achieves a substantial increase in virus yield, with approximately a 2-fold increase in virus titer for multiple serotypes, including AAV2, AAV5, AAV8, and AAV9.

Reduced Usage Cost: By reducing the amount of transfection reagent by 50% while maintaining high yield, the product effectively lowers production costs.

Storage	Hieff Trans™ PEI AAV Transfection Reagent-GMP products should be stored at 2-8℃ for 2 year.
Form	Liquid

<a href="https://cdn.shopify.com/s/files/1/0803/9419/1166/files/40824-Hieff_Trans_TM_Ultra_PEI-AAV_Transfection_Reagent-GMP_Ver.EN20250121_2.pdf?v=1737531224" target="_blank">Manual</a> <a href="https://cdn.shopify.com/s/files/1/0803/9419/1166/files/40824--MSDS-HB250121.pdf?v=1737531224" target="_blank">Safety<br />rnData Sheet</a>

No Description Available.

Hieff Trans™ PEI AAV showing ultra trans-effciency compared with top PEIs. AAV2, AAV5, AAV8, and AAV9 were produced in suspension 293F cells, with a DNA dosage of 1 µg per million cells. The virus was harvested 72 hours post-transfection, and the viral supernatant was analyzed.

Hieff Trans™ PEI showing low inputs. AAV9 was produced in suspension 293F cells with different input of Ultra-PEI (Left, Plasmids input: 0.5 μg) or Plasmids (Right, Ultra-PEI input 0.6 μL) dosages per million cells. The virus was harvested 72 hours post-transfection.

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SKU	Supplier No.	Size	Your price	Quantity
BHM20800020	40824ES, 40824ES10	10mL	$7555		Add to Cart

Hieff Trans™ UltraAAV Transfection Reagent (PEI, GMP grade) _ 40824ES

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