UCF.ME^TM rTEV Protease(5 U/uL)

Recombinant TEV protease (rTEV Protease) is a recombinant protease modified and purified by genetic engineering, which not only maintains the functional activity of natural TEV Protease, but also shows stronger stability and specificity over a broad temperature range. rTEV Protease is a commonly tool enzyme for cutting affinity labels on fusion protein. It is specific to the seven amino acid sequence EXXYXQ?(G/S), and the cutting site is between glutamine and glycine/serine. The commonly used seven amino acid sequence is Glu-Asn-Leu-Tyr-Phe-Gln?-Gly. rTEV Protease is optimally active at pH 7.0, 30°C, but is active over a wide range of pH 6.0-8.5 and temperatures 4-30°C (see Table 1), allowing the choice of reaction conditions to be flexible depending on the target protein. In addition, the 6×His label of N-terminal can also be used to remove rTEV Protease by Ni-NTA resin to purify the target protein.

The product has been optimized on the basis of 20403ES, and compared with 20403ES, it has higher purity, stronger activity and lower E.coli gDNA residue, which is suitable for application with higher requirements for enzyme purity, activity and residue.

Features

High cleavage efficiency: The protein tag is removed more thoroughly.

Low residual host gDNA: Effectively reduce the risk of introducing exogenous substance residues.

Applications

Removal of fusion tags

Specifications

Components

Shipping and Storage

rTEV Protease, stored at -25~-15^oC for 6 months; Store at -85~-65^oC for 1 year.

rTEV Buffer, stored at -25~-15^oC for 1 year.

Note: It is recommended to store each component separately when receiving the goods or using it for the first time to avoid repeated freezing and thawing.

Figures

Figure 1. Verification of Cleavage Activity of UCF.ME^TM rTEV Protease

Note: Using a fusion protein (3 ?g) containing the UCF.ME^TM rTEV Protease cleavage site as the substrate, UCF.ME^TM rTEV Protease was added at 10, 5, and 3 U, respectively. The reaction was carried out at 30 ^oC for 1 hour, and the cleavage effect was detected by agarose gel electrophoresis. The results showed that when the amount of UCF.ME^TM rTEV Protease was more than 3 U, it could cleave the substrate efficiently with a cleavage efficiency of >90%.

Documents:      

Safety Data Sheet

20427_MSDS_Ver.EN20250120.pdf

Manuals

20427_Manual_Ver.EN20240722.pdf