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Home / VECTOR &VIRUS / Lentivirus

LV-CMV-mCherry-CD63(m)-WPRE

SKU : BHV12400991

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Description
Review(s)

Mfr.No.	LV-0966
Promoter	CMV
Insert Gene Name	A modified version of CD63
Fluorophore/tag	mcherry
Description	The "LV-CMV-EGFP-CD63(h)-WPRE" vector is designed for efficient gene delivery, high-level expression, and easy tracking of transduced cells in human cell-based research. It combines the strong CMV promoter, the visual marker EGFP, and the human form of the CD63 gene, all enhanced by the WPRE for increased expression. This vector is particularly useful in studies involving cellular membrane dynamics, exosome research, and other areas where CD63 plays a key role. It has the following gene components and functions: LV (Lentiviral): This denotes that the vector is based on lentivirus technology, which is efficient in delivering genetic material into both dividing and non-dividing cells. Lentiviruses are known for their ability to integrate the delivered genes into the host cell genome, ensuring stable and long-term expression. CMV (Cytomegalovirus Promoter): A highly active and commonly used viral promoter in gene therapy and molecular biology. It drives robust and constitutive expression of the downstream genes in a wide range of mammalian cells. This promoter is known for its strong transcriptional activity, which makes it suitable for high-level expression of the inserted genes. EGFP (Enhanced Green Fluorescent Protein): A fluorescent protein that emits bright green fluorescence when exposed to light in the blue to ultraviolet range. EGFP is used as a reporter gene, allowing researchers to visually track and monitor the expression of the gene of interest and the efficiency of transduction in live cells. Its fluorescence provides a convenient means of identifying and sorting transduced cells. CD63(h): This component represents the human version of the CD63 gene. CD63 is a member of the tetraspanin family, typically located on the surface of cellular membranes, including exosomes. It plays a role in various cellular processes such as cell adhesion, migration, and signaling. The "(h)" notation indicates that this CD63 gene is specifically the human isoform, making the vector suitable for human cell research and potentially more relevant for applications in human biology and medicine. WPRE (Woodchuck hepatitis virus Posttranscriptional Regulatory Element): A genetic element that enhances the mRNA transcript stability of the inserted gene, leading to higher levels of gene expression in the transduced cells. The inclusion of WPRE is a common strategy in vector design to boost the overall efficiency of gene expression, ensuring that the transgene is expressed at sufficient levels for detection and functional analysis.
Broad Neuronal specific	Broad
Function	Other
Gene Alt Name	CD63(m)
Production System	The third generation of lentiviral (LV) production systems
Quality Control Tests	qPCR and detection of cell fluorescence
Virus Type	Mammalian Expression, Lentiviral
Purification	high speed centrifugation
Storage	4°C for short-term storage and -80°C for long-term storage; each freeze-thaw cycle reduces virus titer by 10%, repeated freeze-thaws should be avoided. When storing the virus at -80°C for more than 6 months, it is recommended to re-determine the viral titer or conduct pre-experimental tests of product activity before use.

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Combination SKU	Supplier No.	Size	Titer	Your price	Quantity
BHV12400991-1	LV-0966	1ml	>=5e+8 TU/ml	$755		Add to Cart
BHV12400991-2	LV-0966	2ml	>=5e+8 TU/ml	$1356		Add to Cart
BHV12400991-3	LV-0966	5ml	>=5e+8 TU/ml	$2897		Add to Cart
BHV12400991-4	LV-0966	500ul	>=1e+9 TU/ml	$895		Add to Cart
BHV12400991-5	LV-0966	1ml	>=1e+9 TU/ml	$1599		Add to Cart
BHV12400991-6	LV-0966	2ml	>=1e+9 TU/ml	$2900		Add to Cart
BHV12400991-7	LV-0966	5ml	>=1e+9 TU/ml	$4400		Add to Cart

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