{"product_id":"1-kb-plus-dna-ladder-bhn15200005","title":"1 kb Plus DNA Ladder","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Ladders \u0026amp; Markers\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e1 kb Plus DNA Ladder (1 kb plus Ladder DNA Marker) is composed of 13 DNA fragments with a length of 250 bp to 12,000 bp, dissolved in 1× Loading Buffer, and can be loaded directly, 5–10 µL per well, with no further preparation.\u003c\/p\u003e\n\u003cp\u003eThe thirteen fragments are 250, 500, 750, 1,000, 1,500, 2,000, 2,500, 3,000, 4,000, 5,000, 6,000, 8,000 and 12,000 bp. The 1,500 bp and 4,000 bp indicator bands contain 100 ng per 5 µL and every other band contains 40 ng per 5 µL. The marker is ready to use and needs no added loading buffer. A gel concentration of 1%–2% agarose is recommended.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFragment range\u003c\/th\u003e\n\u003ctd\u003e250–12,000 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNumber of bands\u003c\/th\u003e\n\u003ctd\u003e13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBand sizes\u003c\/th\u003e\n\u003ctd\u003e250, 500, 750, 1,000, 1,500, 2,000, 2,500, 3,000, 4,000, 5,000, 6,000, 8,000 and 12,000 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReference band(s)\u003c\/th\u003e\n\u003ctd\u003e1,500 bp and 4,000 bp indicator bands at 100 ng per 5 µL; other bands 40 ng per 5 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eReady-to-use; premixed in 1× Loading Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended load\u003c\/th\u003e\n\u003ctd\u003e5–10 µL per well; 5 µL for wells narrower than 5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended gel\u003c\/th\u003e\n\u003ctd\u003e1%–2% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSizing of DNA fragments in agarose gel electrophoresis\u003c\/li\u003e\n\u003cli\u003eDirect loading as a size reference alongside samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eTwo optimized Loading Buffer dyes are added without blocking the brightness of DNA band fluorescence.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C with a shelf life of two years.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLoad 5 µL per run when the sample well is narrower than 5 mm; increase the volume for wider wells.\u003c\/li\u003e\n\u003cli\u003eAgarose purity strongly affects the clarity of DNA bands, so the best quality agarose should be used.\u003c\/li\u003e\n\u003cli\u003eThe recommended gel concentration is 1%–2%.\u003c\/li\u003e\n\u003cli\u003eHigher agarose concentration improves separation of short DNA fragments, while lower concentration favours separation of long fragments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEDL108\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHN15200005\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"500 uL","offer_id":53965366559085,"sku":"EDL108-500UL","price":0.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":53965366853997,"sku":"EDL108-5ML","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EDL108-a-1.jpg?v=1789414949","url":"https:\/\/www.ebiohippo.com\/products\/1-kb-plus-dna-ladder-bhn15200005","provider":"BioHippo","version":"1.0","type":"link"}