{"product_id":"10-x-h19-dilution-buffer-bht16300178","title":"10 x H19 Dilution Buffer","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eAssay Buffers\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e10 x H19 Dilution Buffer is the diluent for the H19 Dye used in DNA relaxation and supercoiling assays. It is the same dilution buffer used in the ProFoldin DNA relaxation and supercoiling assay kits and is sold separately for running additional assays. 100 ml makes 1 L of 1 x buffer, enough for about 4,000 assays at 250 µl of diluted dye per well.\u003c\/p\u003e\n\u003cp\u003eDilute the 10 x buffer 10-fold with water to make 1 x H19 Dilution Buffer, then dilute the 1500 x H19 dye in it (1 µl of dye per 1.5 ml) just before use. After the enzyme reaction, 250 µl of diluted dye is added to each 40 µl reaction and fluorescence is read at Ex 485 nm \/ Em 535 nm.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e100 ml (10 x) – H19BUF-10\u003c\/th\u003e\n\u003ctd\u003e100 ml of 10 x buffer for the H19 Dye for DNA relaxation and supercoiling assays\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e10 x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003ctd\u003e100 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUse\u003c\/th\u003e\n\u003ctd\u003eDiluent for the H19 Dye in DNA relaxation and supercoiling assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eH19BUF-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe ProFoldin protocol “H19 Dye for DNA Relaxation and Supercoiling Assays” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 ml (10 x)","offer_id":67828368343405,"sku":"H19BUF-10","price":199.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/10-x-h19-dilution-buffer-bht16300178","provider":"BioHippo","version":"1.0","type":"link"}