{"product_id":"16s-v4-bacterial-ampseq-prep-kit-for-mgi-bht20800026","title":"16S (V4) Bacterial AmpSeq Prep Kit For MGI","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp align=\"justify\"\u003eThe 16S (V4) Bacterial Amplicon Library Preparation Kit enables high-throughput sequencing-based amplification of the V4 region of the 16S rDNA gene. Combined with bioinformatics analysis, this kit allows detection of microorganisms (primarily bacteria) in specific environmental samples, enabling identification of bacterial species and providing comprehensive information on taxonomic classification, species abundance, community structure, phylogenetic relationships, and more.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThis kit includes all reagents required for bacterial 16S rDNA amplification. The 16S (V4) primer mix (F\/R) amplifies the first ~300 bp fragment of the bacterial 16S rDNA gene. For full-length library construction compatible with MGI™ sequencing platforms, this kit must be used in combination with the Hieff NGS™ Unique Dual Barcode Primer Kit for MGI(Cat# 13350–13353).\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eAll reagents provided in this kit have undergone stringent quality control and functional validation to ensure maximum stability and reproducibility during library preparation.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThe 16S rRNA gene is located in the small subunit of the prokaryotic ribosome and consists of 10 conserved regions and 9 hypervariable regions. The conserved regions exhibit minimal variation across bacterial taxa, whereas the hypervariable regions are genus- or species-specific and vary according to phylogenetic relatedness. Therefore, the 16S rDNA sequence serves as a characteristic molecular marker for species identification and is widely regarded as the optimal target for bacterial phylogenetic analysis and taxonomic classification.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e16S rDNA amplicon sequencing typically involves selecting one or more hypervariable regions and designing universal primers within flanking conserved regions for PCR amplification, followed by sequencing and analysis of the variable regions for microbial identification. This approach has become a cornerstone method for characterizing microbial community composition in environmental samples. Amplification of the V4 region of 16S rDNA from diverse environmental DNA samples enables discrimination and identification of the vast majority of bacterial taxa and represents the most commonly used standard for bacterial classification.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003eBroad Sample Compatibility:\u003c\/strong\u003e Supports metagenomic samples ranging from 100 pg to 10 ng, including feces, soil, and extracted meta-DNA.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003eHigh Amplification Efficiency:\u003c\/strong\u003e Validated across multiple samples, delivering excellent library yields and high-quality sequencing data.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003cstrong\u003eConsistent Quality:\u003c\/strong\u003e Rigorous batch-to-batch performance and stability controls ensure reliable results.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"9\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"160\" valign=\"center\" colspan=\"2\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"296\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12933ES08\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12933ES24\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"130\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12933ES96\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"112\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12933-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"47\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_18a10826-8c6f-45f5-bdb1-3d6f37e1a76f.png?v=1764300019\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"296\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e2×Hieff™ Multiplex PCR Master Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e300 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"130\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e1200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"112\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12933-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"47\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_e7f8f547-e52f-4d81-9b9a-2c8fb50328e2.png?v=1764300019\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"296\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e16S Primer Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e16 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e48 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"130\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e192 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"112\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e12933-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"47\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_6a374fd4-c606-4e3f-b5c5-ee4a946ff489.png?v=1764300021\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"296\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e2×Hieff Canace™ Amplification Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e100 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"123\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e300 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"130\" valign=\"center\"\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e1200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003eComponent 12933-A: Store at \u003cspan data-type=\"text\"\u003e\u003cspan data-type=\"leaf\"\u003e-25℃~-15℃\u003c\/span\u003e\u003c\/span\u003e;\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eAll other components: Store at –25 ~ –15 °C\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eValid life: 1 year\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eMicrobiome Research; Microbial Community Analysis; Environmental Surveillance, Clinical Microbiology; Agriculture-related Microbial Investigations.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e1.Workflow \u003c\/strong\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_86536ed2-7c15-4982-84d5-f9edc2e31164_1024x1024.png?v=1764300657\" alt=\"Figure 1.Amplicon Library Preparation Workflow\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 1.Amplicon Library Preparation Workflow\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eHigh Amplification Efficiency\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_04843a39-0100-4f12-aef9-467d437079b2_1024x1024.png?v=1764300660\" alt=\"Figure 1. Amplification performance of the 16S-V4-MGI workflow using 10 ng DNA from soil, fecal, and bacterial samples. First PCR: 25 µL, 28 cycles; second PCR: 100 ng first-round product, 6 cycles. All samples produced successful amplicons with comparable efficiency using Kit 12933.\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 2. Amplification performance of the 16S-V4-MGI workflow using 10 ng DNA from soil, fecal, and bacterial samples. First PCR: 25 µL, 28 cycles; second PCR: 100 ng first-round product, 6 cycles. All samples produced successful amplicons with comparable efficiency using Kit 12933.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12933-MSDS-HB251128.pdf?v=1764300790\"\u003e12933_MSDS_HB251120_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12933ES-Manual-Ver.EN20251120.pdf?v=1774935483\"\u003e12933_Manual_HB20260327.pdf\u003c\/a\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"24 T","offer_id":53331732267373,"sku":"12933ES24","price":255.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53332190888301,"sku":"12933ES96","price":972.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/3_18a10826-8c6f-45f5-bdb1-3d6f37e1a76f.png?v=1778795252","url":"https:\/\/www.ebiohippo.com\/products\/16s-v4-bacterial-ampseq-prep-kit-for-mgi-bht20800026","provider":"BioHippo","version":"1.0","type":"link"}