{"product_id":"1x-dsdna-hs-assay-kit-bht20800083","title":"1×dsDNA HS Assay kit","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg\/μL to 100 ng\/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eFast:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Ready-to-use premixed solution for easy handling; results available in just 5 minutes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eSensitive:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Accurately quantifies samples ranging from 0.2 to 100 ng.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eAccurate:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Minimal deviation compared to competing products, with all errors controlled within 10% and low batch-to-batch variation.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eStable:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Consistent performance across different production batches.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"17.1600%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"29.4800%\" valign=\"top\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.4000%\" valign=\"top\"\u003e\n\u003cp\u003eConcentration\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5200%\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e12642ES60\u003c\/p\u003e\n\u003cp\u003e(100 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.4000%\" valign=\"top\"\u003e\n\u003cp align=\"center\"\u003e12642ES76\u003c\/p\u003e\n\u003cp\u003e(500 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"17.1600%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12642-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"29.4800%\" valign=\"center\"\u003e\n\u003cp\u003e1×dsDNA HS Working Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.4000%\" valign=\"center\"\u003e\n\u003cp\u003e1×Concentrate in DMSO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5200%\" valign=\"center\"\u003e\n\u003cp\u003e50 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.4000%\" valign=\"center\"\u003e\n\u003cp\u003e250 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"17.1600%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12642-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"29.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003edsDNA Standard 1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.4000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0 ng\/μL in TE buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.4000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5\u003ca name=\"OLE_LINK2\"\u003e\u003c\/a\u003e×1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"17.1600%\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12642-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"29.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003edsDNA Standard 2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"27.4000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 ng\/μL in TE buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.5200%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"12.4000%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5×1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at 2~8℃ away from light for 12 months.\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003edsDNA quantification; DNA concentration determination\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eEasy-to-use and streamlined quantification workflow\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-1_1024x1024.png?v=1751216052\" alt=\"Figure 1. DNA Quantification Workflow\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"justify\" style=\"text-align: center;\"\u003e\u003cb\u003e\u003cspan class=\"16\"\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cstrong\u003eFigure 1. DNA Quantification Workflow\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eGood linearity:  12642 has good linearity in the range of 0.2-100 ng.\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-2_1024x1024.png?v=1751216051\" alt=\"Figure 2. Linear Relationship Test\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eFigure 2. Linear Relationship Test\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eLinear quantification of 11 dsDNA concentrations (0.5–10 ng\/μL) using 1264\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eDye binding specificity\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-3_1024x1024.png?v=1751216051\" alt=\"Figure 3. Specificity test\" style=\"margin-bottom: 16px; float: none; display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"justify\" style=\"text-align: center;\"\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003cstrong\u003eFigure 3. Specificity test\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan class=\"15\"\u003eTest Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan class=\"15\"\u003eConclusion:\u003c\/span\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003cspan class=\"15\"\u003eAdding equal amounts of ssDNA slightly increases the 12642 reading; error \u0026lt;10%\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(left)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e.\u003c\/span\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003e\u003cspan class=\"15\"\u003eAdding equal amounts of RNA also increases the 12642 reading; error \u0026lt;10%\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e(right)\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e4. \u003c!--[endif]--\u003eEfficient dye binding and stable fluorescence signal\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 4. Dye binding efficiency and fluorescence signal persistence\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-4_1024x1024.png?v=1751216051\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\" class=\"p\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cstrong\u003eFigure 4. Dye binding efficiency and fluorescence signal persistence\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eTest Scheme: Two RNA libraries of different concentrations were measured using 12642 on the Qubit 3.0 with a 2 μL sample volume.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eConclusion: The fluorescence signal saturated within 2 minutes and remained stable for up to 5 h.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e5. \u003c!--[endif]--\u003e Strong tolerance to common contaminants\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cb\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-5.png?v=1751216052\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e6. \u003c!--[endif]--\u003eUltra-high stability\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 5. Stability comparison of Yeasen's  (Cat#12642ES) and competitor T brand\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-6_1024x1024.png?v=1751216052\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cstrong\u003eFigure 5. Stability comparison of Yeasen's  (Cat#12642ES) and competitor T brand\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eTest Scheme: Two dsDNA concentrations were measured over time using \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003esupplier \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eT\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e*\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e (4°C), Yeasen#12642 (4°C), and Yeasen#12642 (room temperature, ~25°C). Deviation from the initial value (Day 0) was calculated.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003e\u003cstrong\u003eConclusion: \u003c\/strong\u003eYeasen#12642 remained stable at room temperature for 2 weeks, with deviations \u0026lt;10%.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\u003cstrong\u003eFAQ\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: What is the difference between 12640 and 12642?\u003c\/p\u003e\n\u003cp\u003eA: 12642 is a premixed solution, ready-to-use. It can be directly added to the sample to determine the concentration. There is no need to prepare a dye working solution.\u003c\/p\u003e\n\u003cp\u003eQ: The product composition consists of only two standard substances. When creating the standard curve, is it necessary to perform multiple dilutions on the \"standard 2\" sample?\u003c\/p\u003e\n\u003cp\u003eA: When using Qubit for quantification, only two standards are needed according to the operation steps. If using an enzyme reader, multiple gradients are required to create the standard curve. In this case, 1×TE can be used to gradientally dilute standard 2.\u003c\/p\u003e\n\u003cp\u003eQ: If we replace Qubit detection with an enzyme detector, how should we select the enzyme detection plate and how should the wavelength be set?\u003c\/p\u003e\n\u003cp\u003eA: Select the black microplate. Wavelength: Ex = 480 nm, Em = 520 nm.\u003c\/p\u003e\n\u003cp\u003eQ: Is there a difference between the fluorescence value of the standard sample and the previous ones? What should the typical fluorescence value of a standard sample be?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: The fluorescence value of the standard sample is not fixed, and there may be differences among different instruments. To verify, you can perform multiple replicate wells or use multiple gradients, and if a linear relationship is observed, it indicates success.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eS\u003c\/span\u003eafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-MSDS-HB250619_72dba6fc-de02-47e4-b750-fbd338beba24.pdf?v=1753170931\"\u003e12642_MSDS_HB250619.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12642-Manual-Ver.EN20250619_66580fc2-cbbf-4a25-b136-e557e3273004.pdf?v=1775022426\"\u003e12642_Manual_HB20260401.pdf\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 T","offer_id":53339939537261,"sku":"12642ES60","price":105.0,"currency_code":"USD","in_stock":true},{"title":"500 T","offer_id":53339941372269,"sku":"12642ES76","price":325.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/12642-1_1024x1024_3b96443e-d7b7-44c7-931e-b1b92520f327.png?v=1779018080","url":"https:\/\/www.ebiohippo.com\/products\/1x-dsdna-hs-assay-kit-bht20800083","provider":"BioHippo","version":"1.0","type":"link"}