{"product_id":"20x-sybr-green-dye-bht15200045","title":"20x SYBR Green Dye","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eNucleic Acid Dyes\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe fluorescence signal of SYBR Green Dye combined with dsDNA can be enhanced 800-1000 times. In the PCR reaction system, an excess of SYBR Green Dye fluorescent dye is added. After it binds the double-stranded DNA product, the fluorescent signal is enhanced, while unbound dye molecules remain weakly fluorescent, thereby ensuring that the fluorescence signal increase is completely synchronized with the increase of PCR products. Fluorescence can be measured in the annealing stage or the extension stage.\u003c\/p\u003e\n\u003cp\u003eThe product is a single-component 20× concentrated dye solution for fluorescent qPCR detection. The final working concentration is 0.2×–1×, typically 0.5×, obtained by diluting the concentrate 20–100 times; 0.5–2.5 µL of the 20× concentrate is added to a 50 µL PCR reaction and the concentration is optimized for each application.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSingle-component concentrated dye solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e20× concentrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFinal working concentration\u003c\/th\u003e\n\u003ctd\u003e0.2×–1×, typically 0.5×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended dilution\u003c\/th\u003e\n\u003ctd\u003e20–100 times\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSignal enhancement\u003c\/th\u003e\n\u003ctd\u003e800–1000 times when combined with dsDNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eROX\u003c\/th\u003e\n\u003ctd\u003eNot supplied; calibration required on certain instruments, e.g. ABI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFluorescent quantitative PCR detection\u003c\/li\u003e\n\u003cli\u003eReal-time monitoring of double-stranded DNA accumulation during PCR\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eTransport at room temperature, long-term storage at -20°C, avoid repeated freezing and thawing. It can be placed at 4°C for short-term use. Shipping: Transport at room temperature.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eOptimize the dye concentration; concentrations that are too low give a weak signal and concentrations that are too high inhibit PCR.\u003c\/li\u003e\n\u003cli\u003eSet the magnesium ion concentration 0.5–3 mM higher than in standard PCR.\u003c\/li\u003e\n\u003cli\u003eROX calibration is required on certain instruments, for example ABI systems.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freezing and thawing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ021\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200045\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"5 mL","offer_id":53965513032045,"sku":"EQ021-5ML","price":0.0,"currency_code":"USD","in_stock":true},{"title":"1 mL x 20","offer_id":53965516538221,"sku":"EQ021-1MLX20","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ021-a-1.jpg?v=1789419309","url":"https:\/\/www.ebiohippo.com\/products\/20x-sybr-green-dye-bht15200045","provider":"BioHippo","version":"1.0","type":"link"}