{"product_id":"2x-hieff-hotstart-pcr-genotyping-master-mix-with-dye-bht20800051","title":"2× Hieff™ HotStart PCR Genotyping Master Mix (With Dye)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-family:inherit;color:#333;line-height:1.65;padding:4px 0}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc h3{color:#1a5c58;font-size:1.05em;font-weight:700;margin:18px 0 8px;border-bottom:2px solid #d0dde0;padding-bottom:4px}\n.bhp-desc ul{margin:4px 0 14px 20px;padding:0}\n.bhp-desc ul li{margin-bottom:5px}\n.bhp-desc table{width:100%;border-collapse:collapse;margin:10px 0 16px;font-size:.93em}\n.bhp-desc th{background:#1a5c58;color:#fff;padding:8px 10px;text-align:left;font-weight:600}\n.bhp-desc td{padding:7px 10px;border-bottom:1px solid #e0e0e0;vertical-align:top}\n.bhp-desc tr:nth-child(even) td{background:#f5fafa}\n.bhp-desc .bhp-note{font-size:.88em;color:#666;font-style:italic;margin-top:6px}\n.bhp-desc .bhp-cite{font-size:.85em;color:#555;margin-top:12px;border-top:1px solid #e0e0e0;padding-top:8px}\n.bhp-desc .bhp-cite p{margin:3px 0;line-height:1.5}\n\u003c\/style\u003e\n\u003cp\u003e2× Hieff™ HotStart PCR Genotyping Master Mix is a ready-to-use 2× premix containing Hieff™ HotStart Taq DNA Polymerase, dNTPs, and an optimized buffer system. Only primers and template are needed to begin amplification, enabling high-throughput genotyping with improved reproducibility. The HotStart Taq polymerase is ligand-modified and completely blocked at room temperature; activity is released by a 2–3 minute 95°C activation step compatible with standard PCR programs.\u003c\/p\u003e\n\u003ch3\u003eFeatures\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eReady-to-use 2× premix — add only primers and template\u003c\/li\u003e\n\u003cli\u003eHot Start technology prevents non-specific amplification during reaction assembly\u003c\/li\u003e\n\u003cli\u003eRapid 2–3 min activation at 95°C; compatible with standard PCR protocols\u003c\/li\u003e\n\u003cli\u003eThermostable with extended half-life at 95°C\u003c\/li\u003e\n\u003cli\u003eGenerates PCR products with 3′-dA overhangs for TA cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eInput DNA Amounts\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003cth\u003eRecommended Input\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGenomic DNA\u003c\/td\u003e\n\u003ctd\u003e50–100 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlasmids\u003c\/td\u003e\n\u003ctd\u003e100 pg–20 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ecDNA\u003c\/td\u003e\n\u003ctd\u003e1–5 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePCR Cycling Conditions\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eStep\u003c\/th\u003e\n\u003cth\u003eTemperature\u003c\/th\u003e\n\u003cth\u003eTime\u003c\/th\u003e\n\u003cth\u003eCycles\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eInitial Denaturation\u003c\/td\u003e\n\u003ctd\u003e95°C\u003c\/td\u003e\n\u003ctd\u003e5 min\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDenaturation\u003c\/td\u003e\n\u003ctd\u003e95°C\u003c\/td\u003e\n\u003ctd\u003e30 sec\u003c\/td\u003e\n\u003ctd\u003e35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnnealing\u003c\/td\u003e\n\u003ctd\u003e50–60°C\u003c\/td\u003e\n\u003ctd\u003e30 sec\u003c\/td\u003e\n\u003ctd\u003e35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExtension\u003c\/td\u003e\n\u003ctd\u003e72°C\u003c\/td\u003e\n\u003ctd\u003e30 sec\/kb\u003c\/td\u003e\n\u003ctd\u003e35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal Extension\u003c\/td\u003e\n\u003ctd\u003e72°C\u003c\/td\u003e\n\u003ctd\u003e10 min\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"bhp-cite\"\u003e\n\u003ch3\u003eSelected Citations\u003c\/h3\u003e\n\u003cp\u003e[1] Han P, et al. Knockdown of hnRNPA1 promotes NSCLC metastasis and EMT by regulating alternative splicing of LAS1L exon 9. \u003cem\u003eFront Oncol.\u003c\/em\u003e 2022;12:837248. (IF: 5.738)\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1 mL 100 T","offer_id":53311795069293,"sku":"10108ES03","price":20.0,"currency_code":"USD","in_stock":true},{"title":"5×1 mL 500 T","offer_id":53311796248941,"sku":"10108ES08","price":100.0,"currency_code":"USD","in_stock":true},{"title":"50×1 mL 5000 T","offer_id":53311796281709,"sku":"10108ES50","price":900.0,"currency_code":"USD","in_stock":true},{"title":"100×1 mL 10000 T","offer_id":53311796314477,"sku":"10108ES60","price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10108-x.jpg?v=1778557126","url":"https:\/\/www.ebiohippo.com\/products\/2x-hieff-hotstart-pcr-genotyping-master-mix-with-dye-bht20800051","provider":"BioHippo","version":"1.0","type":"link"}