{"product_id":"3d-atp-luminescent-cell-viability-assay-kit-bht20800101","title":"3D ATP Luminescent Cell Viability Assay Kit","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp align=\"justify\"\u003eATP is the primary energy currency in cells and serves as a reliable indicator of cellular metabolic activity. Its concentration correlates linearly with the number of viable cells, making ATP quantification an effective method for assessing cell viability or counting live cells.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThis kit utilizes the ATP-dependent luciferase-catalyzed oxidation of luciferin to generate a chemiluminescent signal, enabling highly sensitive and quantitative measurement of intracellular ATP levels. Thanks to high-purity luciferin substrate, thermostable luciferase, and an optimized reaction formulation, this reagent exhibits enhanced cell lysis capability—making it particularly suitable for viability assessment in 3D microtissue cultures.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eUpon addition to cultured cells or 3D spheroids, the reagent rapidly lyses cells, releasing ATP and triggering a stable luminescent reaction (as illustrated). The resulting light intensity is directly proportional to the amount of ATP—and thus to the number of viable cells—within a defined range, allowing accurate quantification of live cell numbers.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eConvenient \u0026amp; Rapid:\u003c\/strong\u003e Ready-to-use detection reagent; stable signal and fast readout.\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eHigh Sensitivity:\u003c\/strong\u003e Delivers superior signal-to-noise ratio, especially for 3D microtissue assays.\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eEfficient Workflow:\u003c\/strong\u003e Data can be acquired within 30 minutes or less after reagent addition.\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eStrong Lysis Capability:\u003c\/strong\u003e Optimized formulation ensures efficient disruption of 3D cell aggregates for complete ATP release.\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003cstrong\u003eScalable Throughput:\u003c\/strong\u003e Suitable for both low-sample experiments and high-throughput screening formats.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable cellspacing=\"9\" border=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"167\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"261\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e40212ES10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"123\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e40212ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"126\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e40212ES80\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"167\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e40212\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"261\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eCellProlif-Glo™ 3D ATP Luminescent Cell Viability Assay Kit\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"118\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"123\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e100 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"126\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e10×100 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25℃~-15℃ for one year.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigure\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eApplication in 3D Spheroid Model\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 1. Cell viability analysis in 3D spheroid model using the 3D ATP Luminescent Cell Viability Assay Kit.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_f2eb8703-94a7-4f64-b75d-40eaeffaf00b_1024x1024.png?v=1763004889\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eFigure 1. Cell viability analysis in 3D spheroid model using the CellProlif-Glo™ 3D ATP Luminescent Cell Viability Assay Kit.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eHeLa cells (5,000 cells\/well, 100 μL) were seeded in low-attachment 96-well plates and cultured for 4–6 days. After incubation, 100 μL of detection reagent was added, followed by shaking for 5 min and standing for 25 min. Then 100 μL of the reaction mixture was transferred to a white opaque 96-well plate for luminescence reading using a Nivo_Lum kinetic mode.\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eApplication in Organoid Models.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 2. Application of the 3D ATP Luminescent Cell Viability Assay Kit in different organoid models.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_9347f0ef-6829-45fe-98e7-06b14a39c0a4_1024x1024.png?v=1763004889\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eFigure 2. Application of the CellProlif-Glo™ 3D ATP Luminescent Cell Viability Assay Kit in different organoid models.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe assay was successfully applied to ovarian cancer organoid model I, lung cancer organoid model II, and thyroid cancer organoid model II, demonstrating broad compatibility with various tumor organoid systems.\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eStrong stability\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 3. Stability evaluation of the 3D ATP Luminescent Cell Viability Assay Kit.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/8_30b9a0ee-0737-4e9f-bdef-2a1e0f5e0db1_1024x1024.png?v=1763004889\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eFigure 3. Stability evaluation of the CellProlif-Glo™ 3D ATP Luminescent Cell Viability Assay Kit.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThe kit retained over 90% of its activity after storage at 4 °C for 60 days, and repeated freeze–thaw cycles (up to 10 times) showed negligible effect on product performance.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/40212--MSDS-HB251111.pdf?v=1763010507\"\u003e40212_MSDS_HB251111_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/40212ES-Manual-Ver.EN20251111.pdf?v=1775613974\"\u003e40212_Manual_HB20260407.pdf\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 mL","offer_id":53339938619757,"sku":"40212ES10","price":115.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":53339940749677,"sku":"40212ES60","price":705.0,"currency_code":"USD","in_stock":true},{"title":"10×100 mL","offer_id":53339940782445,"sku":"40212ES80","price":4975.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/6_1024x1024_0a14bb0d-a322-4182-97e6-f028b2bcd17b.png?v=1779018080","url":"https:\/\/www.ebiohippo.com\/products\/3d-atp-luminescent-cell-viability-assay-kit-bht20800101","provider":"BioHippo","version":"1.0","type":"link"}