{"product_id":"4-nitrophenyl-palmitate-bhb21903242","title":"4-Nitrophenyl palmitate","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eEnzyme Substrates\u003c\/span\u003e\u003ch2\u003eCompound Overview\u003c\/h2\u003e\n\u003cp\u003e4-Nitrophenyl palmitate, also known as 4-Nitrophenyl hexadecanoate, p-Nitrophenyl Palmitate, or pNpp, is a chromogenic substrate for lipases and esterases. Enzymatic hydrolysis releases p-nitrophenol from the compound, which can then be measured by colorimetric detection at 410 nm as an indicator of enzymatic activity. It is used to characterize enzymes from various bacterial and mammalian sources, including Burkholderia species and porcine pancreatic lipase\u003csup\u003e[1][2]\u003c\/sup\u003e. It is supplied as a white to off-white solid (C22H35NO4, MW 377.52) at 99.22% purity.\u003c\/p\u003e\n\u003ch2\u003ePhysical \u0026amp; Chemical Properties\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCAS Number\u003c\/th\u003e\n\u003ctd\u003e1492-30-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Formula\u003c\/th\u003e\n\u003ctd\u003eC22H35NO4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003e377.52 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e99.22%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAppearance\u003c\/th\u003e\n\u003ctd\u003eSolid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eColor\u003c\/th\u003e\n\u003ctd\u003eWhite to off-white\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSMILES\u003c\/th\u003e\n\u003ctd\u003eCCCCCCCCCCCCCCCC(OC1=CC=C([N+]([O-])=O)C=C1)=O\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSignaling Pathway\u003c\/th\u003e\n\u003ctd\u003eMetabolic Enzyme\/Protease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSolubility\u003c\/th\u003e\n\u003ctd\u003eIn Vitro: Chloroform: ≥ 100 mg\/mL (264.89 mM)\u003cbr\u003eDMSO: 6.67 mg\/mL (17.67 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) * \"≥\" means soluble, but saturation unknown.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e4°C, protect from light. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eRoom temperature in continental US; may vary elsewhere.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eLiterature Cited\u003c\/h2\u003e\n\u003cp style=\"font-size:14px;color:#555;margin:0 0 10px\"\u003e\u003cem\u003eSources cited in this description and in the In Vitro \u0026amp; In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e [1]. \u003ca target=\"_blank\" rel=\"noopener\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/12441161\/\u0026lt;br\/\u0026gt;\"\u003eGupta N, et al. Simplified para-nitrophenyl palmitate assay for lipases and esterases. Analytical biochemistry. 2002 Dec 1;311(1):98-9. \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e [2]. \u003ca target=\"_blank\" rel=\"noopener\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/19043691\"\u003eWei P, et al. Characterization of two soil metagenome-derived lipases with high specificity for p-nitrophenyl palmitate. Arch Microbiol. 2009;191(3):233-240.\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003e\u003cstrong\u003eFor Research Use Only.\u003c\/strong\u003e Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.\u003c\/p\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"MedChemExpress (MCE)","offers":[{"title":"500 mg","offer_id":67604985315693,"sku":"HY-W013168-500MG","price":30.0,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":67605516353901,"sku":"HY-W013168-1G","price":48.0,"currency_code":"USD","in_stock":true},{"title":"5 g","offer_id":67605516386669,"sku":"HY-W013168-5G","price":117.0,"currency_code":"USD","in_stock":true},{"title":"10 g","offer_id":67605516419437,"sku":"HY-W013168-10G","price":186.0,"currency_code":"USD","in_stock":true},{"title":"25 g","offer_id":67605516452205,"sku":"HY-W013168-25G","price":371.0,"currency_code":"USD","in_stock":true},{"title":"50 g","offer_id":67605516484973,"sku":"HY-W013168-50G","price":593.0,"currency_code":"USD","in_stock":true},{"title":"100 g","offer_id":67605516517741,"sku":"HY-W013168-100G","price":948.0,"currency_code":"USD","in_stock":true},{"title":"1 mL x 10 mM (in DMSO)","offer_id":67605516550509,"sku":"HY-W013168-1MLX10MM","price":33.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hy-w013168.gif?v=1790973151","url":"https:\/\/www.ebiohippo.com\/products\/4-nitrophenyl-palmitate-bhb21903242","provider":"BioHippo","version":"1.0","type":"link"}