{"product_id":"5-hydroxyindole-bhb21300169","title":"5-Hydroxyindole","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 1H-indol-5-ol.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e 5-Indolol\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 1953-54-4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C8H7NO.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;97%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 100 µM - 1 mM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg\/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e 5-HT3 receptors\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e 5-Hydroxyindole is a 5-HT3 receptor allosteric modulator which slows the desensitization of the channel1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e 5-Indolol\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eSerotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. 5-HT3 receptor is a ligand-gated ion channel receptor of the cys-loop channel family. It is a pentamer with five subunits surrounding a central pore1.5-Hydroxyindole (5-indolol) is a synthetic, allosteric modulator of 5-HT3 channels. It has an effective concentration of 100 μM - 1 mM for 5-HT3A. 5-Hydroxyindole was tested in N1E-115 (neuroblastoma cells) and does not seem to elicit 5-HT receptor mediated currents on its own. However, in the presence of 5-hydroxyindole the effect of 5-HT in the same cells was significantly altered. Peak current amplitude was increased and desensitization of the 5-HT3 receptor was slowed down compared to cells solely infused with 5-HT. The effects of 5-hydroxyindole take place rapidly and are quickly reversed on washing with an external solution. Similar results can be achieved when infusing cells with other 5-HT receptor agonists. 5-Hydroxyindole enhances the peak amplitude of inward currents induced by infusion with the various 5-HT3 receptor agonists by 30-94% and slows down desensitization 2.5 - 5 fold. 5-Hydroxyindole can be used as a tool to selectively modify the desensitization of the 5-HT3 receptor2.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"1 g \/ 1","offer_id":53249709244781,"sku":"H-115-1G-1","price":66.0,"currency_code":"USD","in_stock":true},{"title":"250 mg \/ 1","offer_id":53311559500141,"sku":"H-115-250MG-1","price":50.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/H-115_gr.gif?v=1778544428","url":"https:\/\/www.ebiohippo.com\/products\/5-hydroxyindole-bhb21300169","provider":"BioHippo","version":"1.0","type":"link"}