{"product_id":"5-negative-n-n-dimethyl-amiloride-hydrochloride-bhb21300119","title":"5-(N,N-Dimethyl)amiloride hydrochloride","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 3-amino-6-chloro-N-(diaminomethylidene)-5-(dimethylamino)pyrazine-2-carboxamide;hydrochloride.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e DMA, Dimethyl amiloride\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 1214-79-5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C8H13Cl2N7O.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99% (HPLC)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1-100 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e Up to 50 mM in methanol. Centrifuge all product preparations before use (5,000 x g for 1 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Up to 50 mM in methanol. Centrifuge all product preparations before use (5,000 x g for 1 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TRPA1 channel, Na+\/H+ and Na+\/Ca2+ exchangers\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e 5-(N,N-Dimethyl)amiloride hydrochloride blocks TRPA1 channels with an IC50 of 80 µM1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e DMA, Dimethyl amiloride\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003e5-(N,N-Dimethyl)amiloride hydrochloride (DMA) is an amiloride derivative that selectively inhibits Na+\/H+ exchangers in cellular membranes, resulting in the retention of H+ ions and in the decrease of intracellular pH. Amiloride derivatives are commonly used for their diuretic and antihypertensive properties.Several studies have reported that DMA has positive effects in pancreatic β-cells on insulin release as a result of mis-regulation of intracellular pH and enhanced insulin secretion. Thus, amiloride derivatives are promising potential therapeutic agents for Type 2 Diabetes. In addition, positive effects on other cell functions have been demonstrated such as, cell membrane electrical activity, increase in intracellular Ca2+ and retention of K+1. DMA also enables glucose to induce time-dependent potentiation and enables it to occur in situations where it is normally absent1,2. Another study has suggested the possibility of DMA protection of the heart against post-resuscitation myocardial injury3.DMA also has the ability to inhibit the Na+\/Ca2+ exchanger and to increase its currents in rat cardiomyocytes. Experimental trials have showed that DMA increases calcium sensitivity in normal and hypertrophied cardiomyocytes4.DMA also blocks TRPA1 channels5.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249704788333,"sku":"D-165-10MG-1","price":85.0,"currency_code":"USD","in_stock":true},{"title":"100 mg \/ 1","offer_id":53311552749933,"sku":"D-165-100MG-1","price":595.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311552782701,"sku":"D-165-25MG-1","price":184.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311552815469,"sku":"D-165-5MG-1","price":50.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311552848237,"sku":"D-165-50MG-1","price":333.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/D-165_gr.gif?v=1778544417","url":"https:\/\/www.ebiohippo.com\/products\/5-negative-n-n-dimethyl-amiloride-hydrochloride-bhb21300119","provider":"BioHippo","version":"1.0","type":"link"}