{"product_id":"7-chlorokynurenic-acid-bhb21300089","title":"7-Chlorokynurenic acid","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 7-chloro-4-oxo-1H-quinoline-2-carboxylic acid.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e 7-CKA, 7-CTKA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 18000-24-3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C10H6ClNO3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;98%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1-100 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e 100 mM in DMSO. Centrifuge all product preparations before use (10,000 x g for 1 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NMDA receptors\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e 7-Chlorokynurenic acid is an NMDA receptor antagonist that acts at the glycine site with IC50 of 3.4 μM1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e 7-CKA, 7-CTKA\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003ePeptide confirmation\u003c\/h3\u003e \u003cp\u003eConfirmed by amino acid analysis and mass spectrometry\u003c\/p\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003e7-Chlorokynurenic acid (7-CKA), a derivative of kynurenic acid, is a potent and selective antagonist of the N-methyl-D-aspartate (NMDA) receptor. 7-CKA acts and binds with high affinity to the glycine binding site located on the NMDA receptor. This compound has neuroprotective, antinociceptive and anticonvulsive effects lacking the ability to penetrate the blood-brain-barrier and has therapeutic potential in some types of epilepsy1-3. Several studies that conducted radioligand binding experiments showed that 7-chlorokynurenic acid binds to the strychnine-glycine binding site with high affinity (IC50 value of 0.56 μM)5.Several studies have shown that acute administration of 7-CKA can produce rapid antidepressant-like effects without showing addictive potential2. It seems that inhibition of the phospho-glycogen synthase kinase-3β pathway and activation of the mammalian target of rapamycin in the medial prefrontal cortex are connected to the 7-CKA rapid behavioural response2.NMDA receptors play an important role in a variety of cellular processes and brain functions such as synaptic plasticity, addiction and stroke. NMDA receptors mediate several physiological functions including learning and memory formation, they play a role in glutamate excitotoxicity and are involved in many neurodegenerative conditions including Alzheimer's disease4.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249706688877,"sku":"C-285-10MG-1","price":66.0,"currency_code":"USD","in_stock":true},{"title":"100 mg \/ 1","offer_id":53311556944237,"sku":"C-285-100MG-1","price":476.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311556977005,"sku":"C-285-25MG-1","price":145.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311557009773,"sku":"C-285-50MG-1","price":263.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/C-285_gr.gif?v=1778544409","url":"https:\/\/www.ebiohippo.com\/products\/7-chlorokynurenic-acid-bhb21300089","provider":"BioHippo","version":"1.0","type":"link"}